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NEB DNA Polymerase
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Thermophilic DNA Polymerases<br><br> {| width="100%" cellspacing="0" cellpadding="2" border="1" bgcolor="#e7dcc1" align="center" |- | height="30" align="center" | | height="30" | Vent | height="30" align="center" | Vent (exo-) | height="30" align="center" | Deep<br> Vent | height="30" align="center" | Deep<br> Vent<br> (exo-) | height="30" align="center" | Taq | height="30" align="center" | 9°Nm | align="center" | Phusion** | align="center" | LongAmp<br> Taq | align="center" | LongAmp<br> Hot Start<br> Taq | align="center" | OneTaq | align="center" | Phusion<br> Hot Start<br> Flex** |- | height="30" colspan="12" | Exonuclease Activities |- | 3'—>5' proofreading<br> exonuclease | bgcolor="#e5e5e5" align="center" | yes | align="center" | no | bgcolor="#e5e5e5" align="center" | yes | align="center" | no | bgcolor="#e5e5e5" align="center" | no | align="center" | no* | bgcolor="#e5e5e5" align="center" | yes | align="center" | yes | bgcolor="#e5e5e5" align="center" | yes | align="center" | yes | bgcolor="#e5e5e5" align="center" | yes |- | 5'—>3' exonuclease<br> activity | bgcolor="#e5e5e5" align="center" | no | align="center" | no | bgcolor="#e5e5e5" align="center" | no | align="center" | no | bgcolor="#e5e5e5" align="center" | yes | align="center" | no | bgcolor="#e5e5e5" align="center" | no | align="center" | yes | bgcolor="#e5e5e5" align="center" | yes | align="center" | yes | bgcolor="#e5e5e5" align="center" | no |- | height="30" colspan="12" | Thermal Stability |- | Half life at 95°C | bgcolor="#e5e5e5" align="center" | 6.7 hours | align="center" | 6.7 hours | bgcolor="#e5e5e5" align="center" | 23 hours | align="center" | 23 hours | bgcolor="#e5e5e5" align="center" | 0.9 hours | align="center" | 6.7 hours | bgcolor="#e5e5e5" align="center" | >6 hours <br> at 96°C | align="center" | ND | bgcolor="#e5e5e5" align="center" | ND | align="center" | ND | bgcolor="#e5e5e5" align="center" | ND |- | Half life at 100°C | bgcolor="#e5e5e5" align="center" | 1.8 hours | align="center" | 1.8 hours | bgcolor="#e5e5e5" align="center" | 8 hours | align="center" | 8 hours | bgcolor="#e5e5e5" align="center" | <0.1 hours | align="center" | 1.8 hours | bgcolor="#e5e5e5" align="center" | 2 hours <br> at 98°C | align="center" | ND | bgcolor="#e5e5e5" align="center" | ND | align="center" | ND | bgcolor="#e5e5e5" align="center" | ND |- | height="30" colspan="12" | Primer Extension Characteristics |- | Units/100 µl <br> Primer Extension Rxn | bgcolor="#e5e5e5" align="center" | 1-2 units | align="center" | 2-4 units | bgcolor="#e5e5e5" align="center" | 1-2 units | align="center" | 2-4 units | bgcolor="#e5e5e5" align="center" | 2-5 units | align="center" | 1-2 units | bgcolor="#e5e5e5" align="center" | 1-2 units | align="center" | 10 units | bgcolor="#e5e5e5" align="center" | 10 units | align="center" | 2-5 units | bgcolor="#e5e5e5" align="center" | 1-2 units |- | Error Rate <br> (x10-6 base insertions) | bgcolor="#e5e5e5" align="center" | 57 | align="center" | 190 | bgcolor="#e5e5e5" align="center" | ~20 | align="center" | ~190 | bgcolor="#e5e5e5" align="center" | 285 | align="center" | in progress | bgcolor="#e5e5e5" align="center" | 0.44 | align="center" | ~140 | bgcolor="#e5e5e5" align="center" | ~140 | align="center" | ~140 | bgcolor="#e5e5e5" align="center" | 0.44 |- valign="top" | Resulting DNA Ends | valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends | valign="middle" align="center" | 3´ A | valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends | valign="middle" align="center" | 3´ A | valign="middle" bgcolor="#e5e5e5" align="center" | 3´ A | valign="middle" align="center" | 3´ A<br> blunt | valign="middle" bgcolor="#e5e5e5" align="center" | blunt | valign="middle" align="center" | 3´ A<br> blunt | valign="middle" bgcolor="#e5e5e5" align="center" | 3´ A<br> blunt | valign="middle" align="center" | 3´ A<br> blunt | valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends |- valign="top" | Strand Displacement | valign="middle" bgcolor="#e5e5e5" align="center" | yes;<br> temp. <br> dependent | valign="middle" align="center" | yes;<br> temp. <br> dependent | valign="middle" bgcolor="#e5e5e5" align="center" | yes;<br> temp. <br> dependent | valign="middle" align="center" | yes;<br> temp. <br> dependent | valign="middle" bgcolor="#e5e5e5" align="center" | no | valign="middle" align="center" | yes | valign="middle" bgcolor="#e5e5e5" align="center" | no | valign="middle" align="center" | yes;<br> temp. <br> dependent | valign="middle" bgcolor="#e5e5e5" align="center" | yes;<br> temp. <br> dependent | valign="middle" align="center" | yes;<br> temp. <br> dependent | valign="middle" bgcolor="#e5e5e5" align="center" | no |- | Longest Primer <br> Extension to Date | bgcolor="#e5e5e5" align="center" | 13.2 kb | align="center" | 15.0 kb | bgcolor="#e5e5e5" align="center" | 14.0 kb | align="center" | 15.0 kb | bgcolor="#e5e5e5" align="center" | 8.1 kb | align="center" | 14.0 kb | bgcolor="#e5e5e5" align="center" | 37 kb | align="center" | 40 kb | bgcolor="#e5e5e5" align="center" | 40 kb | align="center" | 15.0 kb | bgcolor="#e5e5e5" align="center" | 20 kb |} *1-5% of wild type exonuclease activity is present in the modified enzyme<br><br> Properties of DNA Polymerases {| cellspacing="0" cellpadding="2" border="1" bgcolor="#e7dcc1" align="center" |- | nowrap="nowrap" align="center" | | align="center" | 5'—>3' Exonuclease | align="center" | 3'—>5' Exonuclease | align="center" | Error Rate(x10-6) | align="center" | Strand Displacement | align="center" | Nick Translation | align="center" | Thermal Stability | align="center" | Km dNTPs | align="center" | Km DNA | align="center" | Extend RNA Primer | align="center" | Extension from Nick | align="left" | Primary <br> Applications |- | nowrap="nowrap" | 9°Nm DNA <br> Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 80 µM | nowrap="nowrap" align="center" | 0.05 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Primer Extension |- | nowrap="nowrap" | Bst DNA Polymerase, <br> Full Length | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | |- | nowrap="nowrap" | Bst DNA Polymerase, <br> Large Fragment | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Strand Displacement <br> Applications |- | nowrap="nowrap" | Bsu DNA Polymerase, <br> Large fragment | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" | Labeling, 2nd Strand <br> Synthesis, Strand Displacement |- | nowrap="nowrap" | Crimson Taq <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine) |- | nowrap="nowrap" | Phusion Hot Start<br> Flex DNA Polymerase* | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.44 | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity, hot start) |- | nowrap="nowrap" | Deep Vent<br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 50 µM | nowrap="nowrap" align="center" | 0.01 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity) |- | nowrap="nowrap" | Deep Vent (exo–) <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (long) |- | nowrap="nowrap" | E. coli DNA <br> Polymerase I | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 9 | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 1-2 µM | nowrap="nowrap" align="center" | 5 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Nick Translation |- | nowrap="nowrap" | Klenow Fragment<br> 3´→ 5´ exo– | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 100 | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Labeling |- | nowrap="nowrap" | Klenow Fragment <br> DNA Polymerase I | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18 | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 2 µM | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Polishing Ends |- | nowrap="nowrap" | LongAmp Taq DNA <br> Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | ~140 | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, long) |- | nowrap="nowrap" | LongAmp Hot Start Taq<br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | ~140 | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (hot start, long) |- | nowrap="nowrap" | M-MuLV Reverse <br> Transcriptase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18 µM | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" | cDNA Synthesis |- | nowrap="nowrap" | OneTaq<br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, difficult) |- | nowrap="nowrap" | OneTaq Hot Start<br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (hot start, routine, difficult) |- | nowrap="nowrap" | phi29 DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | +++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.5 µM | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Strand Displacement <br> Applications |- | nowrap="nowrap" | Phusion High-Fidelity <br> DNA Polymerase* | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.44 | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity) |- | nowrap="nowrap" | Sulfolobus DNA <br> Polymerase IV | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" | DNA Synthesis Across <br> Template Lesions |- | nowrap="nowrap" | T4 DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | <1 | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 2 µM | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" | Polishing Ends, <br> 2nd Strand Synthesis |- | nowrap="nowrap" | T7 DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 15 | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18 µM | nowrap="nowrap" align="center" | 18 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" | Site Directed Mutagenesis |- | nowrap="nowrap" | Taq DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 285 | nowrap="nowrap" align="center" | _ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 13 µM | nowrap="nowrap" align="center" | 2 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine) |- | nowrap="nowrap" | Therminator DNA <br> Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Chain Terminator <br> Applications |- | nowrap="nowrap" | Therminator II <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of <br> Modified Nucleotide Analogs |- | nowrap="nowrap" | Therminator III <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of <br> Modfied Nucleotides |- | nowrap="nowrap" | Therminator γ <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | | nowrap="nowrap" align="center" | | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | + | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of gamma-<br> phosphate Labeled deoxynucleotides |- | nowrap="nowrap" | Vent DNA <br> Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 57 | nowrap="nowrap" align="center" | ++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 60 µM | nowrap="nowrap" align="center" | 0.1 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, high fidelity) |- | nowrap="nowrap" | Vent (exo–) <br> DNA Polymerase | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | – | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 190 | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | +++ | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 40 µM | nowrap="nowrap" align="center" | 0.1 nM | nowrap="nowrap" bgcolor="#e5e5e5" align="center" | – | nowrap="nowrap" align="center" | + | nowrap="nowrap" bgcolor="#e5e5e5" | PCR, Sequencing |} {| width="100%" cellpadding="2" cellspacing="0" border="1" bgcolor="#e7dcc1" | height="30" nowrap="nowrap" bgcolor="#e7dcc1" | | height="30" nowrap="nowrap" align="center" | 3'->5' Proofreading | height="30" nowrap="nowrap" align="center" | Strand Displacement | height="30" nowrap="nowrap" align="center" | Primary Applications |- bgcolor="#e8e8e8" | colspan="4" nowrap="nowrap" | Mesophilic DNA Polymerases |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0269.asp phi29 DNA Polymerase] | align="center" | ++++ | align="center" | +++++ | Strand Displacement Applications |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0203.asp T4 DNA Polymerase] | align="center" | +++++ | align="center" | - | Polishing Ends, 2nd Strand Synthesis |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0209.asp DNA Polymerase I] | align="center" | ++ | align="center" | -* | Nick Translation |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0210.asp DNA Polymerase I, Klenow Fragment] | align="center" | ++ | align="center" | ++ | Polishing Ends |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0212.asp Klenow Fragment (3' -> 5' exo-)] | align="center" | - | align="center" | +++ | Labeling |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0274.asp T7 DNA Polymerase (unmodified)] | align="center" | ++++ | align="center" | - | Site Directed Mutagenesis |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0252.asp Terminal Transferase] | align="center" | - | align="center" | NA | 3' terminal Tailing |- bgcolor="#e8e8e8" | colspan="4" nowrap="nowrap" | Thermophilic DNA Polymerases |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-530.asp Phusion® High Fidelity DNA Polymerase] | align="center" | +++++ | align="center" | - | PCR (high fidelity) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-540.asp Phusion® Hot Start High Fidelity DNA Polymerase] | align="center" | +++++ | align="center" | - | Hot Start PCR (high fidelity) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-549.asp PhusionHot® Start II High Fidelity DNA Polymerase] | align="center" | +++++ | align="center" | - | Hot Start PCR (high fidelity) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-120.asp Phire® Hot Start DNA Polymerase] | align="center" | + | align="center" | - | Hot Start PCR (fast yield) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-122.asp Phire® Hot Start II DNA Polymerase] | align="center" | + | align="center" | - | Hot Start PCR (fast yield) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-505.asp DyNAzyme™ EXT DNA Polymerase] | align="center" | + | align="center" | + | PCR (difficult or long) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-504.asp DyNAzyme™ II Hot Start DNA Polymerase] | align="center" | - | align="center" | - | Hot Start PCR |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0267.asp Taq DNA Polymerase] | align="center" | - | align="center" | -* | PCR (routine), Primer Extension |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0323.asp LongAmp Taq DNA Polymerase] | align="center" | + | align="center" | -* | PCR (long, high-fidelity) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0324.asp Crimson Taq DNA Polymerase] | align="center" | - | align="center" | -* | PCR (routine) |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0254.asp VentR DNA Polymerase] | align="center" | +++ | align="center" | ++ | PCR (high fidelity), Primer Extension |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0257.asp VentR (exo-) DNA Polymerase] | align="center" | - | align="center" | +++ | PCR, Sequencing |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0258.asp Deep VentR DNA Polymerase] | align="center" | +++ | align="center" | ++ | PCR (high fidelity), Primer Extension |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0259.asp Deep VentR (exo-) DNA Polymerase] | align="center" | - | align="center" | <p>+++</p> | PCR (long), Primer Extension |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0260.asp 9°Nm DNA Polymerase] | align="center" | + | align="center" | +++ | Primer Extension |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0261.asp Therminator™ DNA Polymerase] | align="center" | - | align="center" | + | Chain Terminator Applications |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0266.asp Therminator™ II DNA Polymerase] | align="center" | - | align="center" | + | Incorporation of modified substrates |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0275.asp Bst DNA Polymerase, Large Fragment] | align="center" | - | align="center" | +++ | Strand Displacement Applications |- bgcolor="#e8e8e8" | colspan="4" nowrap="nowrap" | Other Polymerases |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0253.asp M-MuLV Reverse Transcriptase] | align="center" | - | align="center" | +++ | cDNA Synthesis |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0277.asp AMV Reverse Transcriptase] | align="center" | - | align="center" | +++ | cDNA Synthesis |- | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0276.asp E. coli Poly(A) Polymerase] | align="center" | - | align="center" | NA | 3´ labeling of RNA |- valign="top" | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0255.asp phi6 RNA Polymerase] | nowrap="nowrap" align="center" | - | nowrap="nowrap" align="center" | NA | nowrap="nowrap" | dsRNA Synthesis |- valign="top" | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0251.asp T7 RNA Polymerase] | nowrap="nowrap" align="center" | - | nowrap="nowrap" align="center" | NA | nowrap="nowrap" | High Yield RNA Production |- valign="top" | nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0207.asp SP6 Polymerase] | nowrap="nowrap" align="center" | - | nowrap="nowrap" align="center" | NA | nowrap="nowrap" | <p>RNA Production</p> |}<br> * Degrades displaced strand. [[Category:Knowledgebase]]
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