NEB DNA Polymerase

Thermophilic DNA Polymerases

	Vent	Vent (exo-)	Deep Vent	Deep Vent (exo-)	Taq	9°Nm	Phusion**	LongAmp Taq	LongAmp Hot Start Taq	OneTaq	Phusion Hot Start Flex**
Exonuclease Activities
3'—>5' proofreading exonuclease	yes	no	yes	no	no	no*	yes	yes	yes	yes	yes
5'—>3' exonuclease activity	no	no	no	no	yes	no	no	yes	yes	yes	no
Thermal Stability
Half life at 95°C	6.7 hours	6.7 hours	23 hours	23 hours	0.9 hours	6.7 hours	>6 hours at 96°C	ND	ND	ND	ND
Half life at 100°C	1.8 hours	1.8 hours	8 hours	8 hours	<0.1 hours	1.8 hours	2 hours at 98°C	ND	ND	ND	ND
Primer Extension Characteristics
Units/100 µl Primer Extension Rxn	1-2 units	2-4 units	1-2 units	2-4 units	2-5 units	1-2 units	1-2 units	10 units	10 units	2-5 units	1-2 units
Error Rate (x10-6 base insertions)	57	190	~20	~190	285	in progress	0.44	~140	~140	~140	0.44
Resulting DNA Ends	blunt ends	3´ A	blunt ends	3´ A	3´ A	3´ A blunt	blunt	3´ A blunt	3´ A blunt	3´ A blunt	blunt ends
Strand Displacement	yes; temp. dependent	yes; temp. dependent	yes; temp. dependent	yes; temp. dependent	no	yes	no	yes; temp. dependent	yes; temp. dependent	yes; temp. dependent	no
Longest Primer Extension to Date	13.2 kb	15.0 kb	14.0 kb	15.0 kb	8.1 kb	14.0 kb	37 kb	40 kb	40 kb	15.0 kb	20 kb

• 1-5% of wild type exonuclease activity is present in the modified enzyme
  
  Properties of DNA Polymerases

	5'—>3' Exonuclease	3'—>5' Exonuclease	Error Rate(x10-6)	Strand Displacement	Nick Translation	Thermal Stability	Km dNTPs	Km DNA	Extend RNA Primer	Extension from Nick	Primary Applications
9°Nm DNA Polymerase	–	+		+++	–	++++	80 µM	0.05 nM	–	+	Primer Extension
Bst DNA Polymerase, Full Length	+	–		_	+	+			+	+
Bst DNA Polymerase, Large Fragment	–	–		++++	–	+			+	+	Strand Displacement Applications
Bsu DNA Polymerase, Large fragment	–	–		++	–	–					Labeling, 2nd Strand Synthesis, Strand Displacement
Crimson Taq DNA Polymerase	+	–		_	+	++			–	+	PCR (routine)
Phusion Hot Start Flex DNA Polymerase*	–	++++	0.44	–	–	+++			–	–	PCR (high fidelity, hot start)
Deep Vent DNA Polymerase	–	+++		++	–	++++	50 µM	0.01 nM	–	+	PCR (high fidelity)
Deep Vent (exo–) DNA Polymerase	–	–		+++	–	++++			–	+	PCR (long)
E. coli DNA Polymerase I	+	++	9	_	+	–	1-2 µM	5 nM	+	+	Nick Translation
Klenow Fragment 3´→ 5´ exo–	–	–	100	+++	–	–			+	+	Labeling
Klenow Fragment DNA Polymerase I	–	++	18	++	–	–	2 µM		+	+	Polishing Ends
LongAmp Taq DNA Polymerase	+	++	~140	_	+	++			–	+	PCR (routine, long)
LongAmp Hot Start Taq DNA Polymerase	+	++	~140	_	+	++			–	+	PCR (hot start, long)
M-MuLV Reverse Transcriptase	–	–		+++	–	–	18 µM				cDNA Synthesis
OneTaq DNA Polymerase	+	++		_	+	++			–	+	PCR (routine, difficult)
OneTaq Hot Start DNA Polymerase	+	++		_	+	++			–	+	PCR (hot start, routine, difficult)
phi29 DNA Polymerase	–	++++		+++++	–	–	0.5 µM		+	+	Strand Displacement Applications
Phusion High-Fidelity DNA Polymerase*	–	++++	0.44	–	–	+++			–	+	PCR (high fidelity)
Sulfolobus DNA Polymerase IV	–	–		–	–	+					DNA Synthesis Across Template Lesions
T4 DNA Polymerase	–	++++	<1	–	–	–	2 µM		+	–	Polishing Ends, 2nd Strand Synthesis
T7 DNA Polymerase	–	++++	15	–	–	–	18 µM	18 nM	+	–	Site Directed Mutagenesis
Taq DNA Polymerase	+	–	285	_	+	++	13 µM	2 nM	–	+	PCR (routine)
Therminator DNA Polymerase	–	–		+	–	++++			+	+	Chain Terminator Applications
Therminator II DNA Polymerase	–	–		+	–	++++			+	+	Incorporation of Modified Nucleotide Analogs
Therminator III DNA Polymerase	–	–		+	–	++++			+	+	Incorporation of Modfied Nucleotides
Therminator γ DNA Polymerase	–	–		+	–	++++			+	+	Incorporation of gamma- phosphate Labeled deoxynucleotides
Vent DNA Polymerase	–	++	57	++	–	+++	60 µM	0.1 nM	–	+	PCR (routine, high fidelity)
Vent (exo–) DNA Polymerase	–	–	190	+++	–	+++	40 µM	0.1 nM	–	+	PCR, Sequencing

	3'->5' Proofreading	Strand Displacement	Primary Applications
Mesophilic DNA Polymerases
phi29 DNA Polymerase	++++	+++++	Strand Displacement Applications
T4 DNA Polymerase	+++++	-	Polishing Ends, 2nd Strand Synthesis
DNA Polymerase I	++	-*	Nick Translation
DNA Polymerase I, Klenow Fragment	++	++	Polishing Ends
Klenow Fragment (3' -> 5' exo-)	-	+++	Labeling
T7 DNA Polymerase (unmodified)	++++	-	Site Directed Mutagenesis
Terminal Transferase	-	NA	3' terminal Tailing
Thermophilic DNA Polymerases
Phusion® High Fidelity DNA Polymerase	+++++	-	PCR (high fidelity)
Phusion® Hot Start High Fidelity DNA Polymerase	+++++	-	Hot Start PCR (high fidelity)
PhusionHot® Start II High Fidelity DNA Polymerase	+++++	-	Hot Start PCR (high fidelity)
Phire® Hot Start DNA Polymerase	+	-	Hot Start PCR (fast yield)
Phire® Hot Start II DNA Polymerase	+	-	Hot Start PCR (fast yield)
DyNAzyme™ EXT DNA Polymerase	+	+	PCR (difficult or long)
DyNAzyme™ II Hot Start DNA Polymerase	-	-	Hot Start PCR
Taq DNA Polymerase	-	-*	PCR (routine), Primer Extension
LongAmp Taq DNA Polymerase	+	-*	PCR (long, high-fidelity)
Crimson Taq DNA Polymerase	-	-*	PCR (routine)
VentR DNA Polymerase	+++	++	PCR (high fidelity), Primer Extension
VentR (exo-) DNA Polymerase	-	+++	PCR, Sequencing
Deep VentR DNA Polymerase	+++	++	PCR (high fidelity), Primer Extension
Deep VentR (exo-) DNA Polymerase	-	+++	PCR (long), Primer Extension
9°Nm DNA Polymerase	+	+++	Primer Extension
Therminator™ DNA Polymerase	-	+	Chain Terminator Applications
Therminator™ II DNA Polymerase	-	+	Incorporation of modified substrates
Bst DNA Polymerase, Large Fragment	-	+++	Strand Displacement Applications
Other Polymerases
M-MuLV Reverse Transcriptase	-	+++	cDNA Synthesis
AMV Reverse Transcriptase	-	+++	cDNA Synthesis
E. coli Poly(A) Polymerase	-	NA	3´ labeling of RNA
phi6 RNA Polymerase	-	NA	dsRNA Synthesis
T7 RNA Polymerase	-	NA	High Yield RNA Production
SP6 Polymerase	-	NA	RNA Production

• Degrades displaced strand.