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Protein Expression Clone Screening
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== E coli Expression Screening 2012 == sfGFP {| cellspacing="1" cellpadding="1" border="1" style="width: 750px;" |- | Clone ID | Protein Name | Vector | Host | Condition | Expression Level | Solubility | Gel Image (Link) | Date | By |- | 360<br> | MBP<br> | pDB.His<br> | Nico21(DE3)plysN<br> | 3ml,37,0.2mMIPTG,5h<br> | high<br> | yes<br> | [[MBP,MMLV,UDG Protein Screening]]<br> | 11/7/2012<br> | yingying<br> |- | [[1428]]<br> | MBP<br> | pT7<br> | Nico21(DE3)plysN<br> | 2ml,37,0.2mMIPTG,5h<br> | no<br> | no<br> | [[PT7-MBP-1428,pT7A3-MBP-1429,Nov,2,2012]]<br> | 11/2/2012<br> | yingying<br> |- | [[1429-pT7A3-MBP|1429]]<br> | MBP<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 3ml,37,0.2mMIPTG,5h<br> | high<br> | yes<br> | [[MBP,MMLV,UDG Protein Screening]] [[PT7-MBP-1428,pT7A3-MBP-1429,Nov,2,2012]]<br> | 11/2/2012 11/7/2012<br> | yingying<br> |- | [[501]]<br> | MMLV<br> | PET30b<br> | Nico21(DE3)plysN<br> | 3ml,37,0.2mMIPTG,5h<br> | no | no<br> | [[MBP,MMLV,UDG Protein Screening]]<br> | 11/7/2012<br> | yingying<br> |- | [[1034]]<br> | UDG<br> | PET8H<br> | Nico21(DE3)plysN<br> | 3ml,37,0.2mMIPTG,5h<br> | yes<br> | no<br> | [[MBP,MMLV,UDG Protein Screening]]<br> | 11/7/2012<br> | yingying<br> |- | [[1375-pDB.His.MBP.3C|1375]]<br> | MBP-3C<br> | pDB-His<br> | Nico21(DE3)plysN<br> | 1L,37, 0.3mMIPTG,4h <br> | yes,only MBP, not 3C<br> | Yes<br> | [[PDB.His.MBP-3C]]<br> | 10/15/2012<br> | Xiuli<br> |- | [[1308-pET-6H-Penetratin-Cre|1308]]<br> | CRE<br> | pET6H<br> | Nico21(DE3)plysN<br> | 2ml,37,0.5mMIPTG,4h <br> | Yes, but leaky, expressed in uninduced <br> | Yes<br> | [[PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre]]<br> | <br> | Xiuli<br> |- | [[1311-pET-6H-VPMLK-TAT-NLS-Cre|1311]]<br> | CRE<br> | pET6H<br> | Nico21(DE3)plysN <br> | 2ml,37,0.5mMIPTG,4h <br> | High<br> | Yes<br> | [[PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre]]<br> | <br> | Xiuli<br> |- | 1307<br> | CRE<br> | pET6H<br> | Nico21(DE3)plysN <br> | 2ml,37,0.5mMIPTG,4h<br> | Faint, need recheck<br> | Yes<br> | [[1307-1309-1310 screening]]<br> | 11/7/2-12<br> | Xiuli<br> |- | [[1310-pET-6H-VPMLK-NLS-Cre|1310]]<br> | CRE<br> | pET6H<br> | Nico21(DE3)plysN <br> | 2ml,37,0.5mMIPTG,4h<br> | High<br> | Yes<br> | [[1307-1309-1310 screening]]<br> | 11/7/2012<br> | Xiuli<br> |- | [[1309-pET-6H-KLPVM-NLS-Cre|1309]]<br> | CRE<br> | pET6H<br> | Nico21(DE3)plysN <br> | 2ml,37,0.5mMIPTG,4h<br> | High<br> | Yes<br> | [[1307-1309-1310 screening]]<br> | 11/7/2012<br> | Xiuli<br> |- | [[1143-1151-1186-screening|1143]]<br> | Exo-1<br> | pET8H<br> | Nico21(DE3)plysN <br> | 3 ml, 37, 0.5mM IPTG, O/N<br> | no<br> | no<br> | [[1143-1151-1186-screening]]<br> | 11/16/2012<br> | Xiuli<br> |- | [[1143-1151-1186-screening|1151]]<br> | sso7d ToughTag, <br> | pET30b<br> | Nico21(DE3)plysN <br> | 3 ml, 37,0.5mM IPTG<br> O/N | no<br> | no<br> | [[1143-1151-1186-screening]]<br> | 11/16/2012<br> | Xiuli<br> |- | [[1143-1151-1186-screening|1155]]<br> | Ara-S1<br> | pET28b<br> | Nico21(DE3)plysN <br> | 3 ml,37,0.5mM IPTG<br> O/N | no<br> | no<br> | [[1143-1151-1186-screening]]<br> | 11/16/2012<br> | Xiuli<br> |- | [[1143-1151-1186-screening|1156]]<br> | Vent Exo<br> | p8H<br> | Nico21(DE3)plysN <br> | 3 ml, 37,0.5mM IPTG<br> O/N | Yes<br> | Yes<br> | [[1143-1151-1186-screening]]<br> | 11/16/2012<br> |- | [[1143-1151-1186-screening|1186]]<br> | MBP-Xis<br> | pDB.Xis<br> | Nico21(DE3)plysN <br> | 3 ml, 37,0.5mM IPTG, O/N | Yes<br> | Yes<br> | [[1143-1151-1186-screening]]<br> | 11/16/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1140]]<br> | <br> polyAPol<br> | pTXB1<br> | Nico21(DE3)plysN<br> | 3 ml, 37,0.5mM IPTG<br> 4 hs | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1325]]<br> | MBP-gpI3L<br> | pDBHis<br> | Nico21(DE3)plysN<br> | 3 ml,37,0.5mM IPTG<br> 4 hsYes | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1225]]<br> | T7-Endo1<br> | pET28b<br> | Nico21(DE3)plysN<br> | 3 ml, 37,0.5mM IPTG<br> O/N | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1206]]<br> | recT<br> | pET28b<br> | Nico21(DE3)plysN<br> | 3 ml,37,0.5mM IPTG, O/N | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1203]]<br> | <br> scIHF2 <br> | pET42b<br> | Nico21(DE3)plysN<br> | 3 ml, 37,0.5mM IPTG<br> O/N | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1245]]<br> | <br> PfuMBP.scIHF2 <br> | pDBHIS<br> | Nico21(DE3)plysN<br> | 3 ml, 37,0.5mM IPTG<br> O/N | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1249]]<br> | <br> Taq_d289<br> | pET<br> | Nico21(DE3)plysN<br> | 3 ml, 37,0.5mM IPTG<br> O/N | No<br> | no<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1140 1325 1225 1206 1203 1245 1249 1464|1464]]<br> | <br> T7 ligase<br> | pT7A3<br> | Nico21(DE3)plysN<br> | <br> | Yes<br> | Yes<br> | [[1140 1325 1225 1206 1203 1245 1249 1464]]<br> | 11/30/2012<br> | Xiuli<br> |- | [[1137-1121-1291-1252-1206|1137]]<br> | Exo-VIII<br> | p8H<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG<br> 4 hrs | No<br> | No<br> | [[1137-1121-1291-1252-1206]]<br> | 12/6/2012<br> | Xiuli<br> |- | [[1137-1121-1291-1252-1206|1121]]<br> | Exo-1<br> | pET28b<br> | Nico21(DE3)plysN<br> | 3ml,37,0.5mM IPTG<br> 4hrs | No<br> | No<br> | [[1137-1121-1291-1252-1206]] | 12/6/2012<br> | Xiuli<br> |- | [[1137-1121-1291-1252-1206|1291]]<br> | TAT-NLS-sso7d<br> | pET30b<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG<br> 4hrs | Yes<br> | Yes<br> | [[1137-1121-1291-1252-1206]]<br> | 12/6/2012<br> | Xiuli<br> |- | [[1137-1121-1291-1252-1206|1252]]<br> | hLysozyme<br> | pTXB1<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG<br> 4 hrs | Yes<br> | No<br> | [[1137-1121-1291-1252-1206]]<br> | 12/6/2012<br> | Xiuli<br> |- | [[1137-1121-1291-1252-1206|1206]]<br> | <br> recT<br> | pET28b<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG 4 hrs | No<br> | No<br> | [[1137-1121-1291-1252-1206]]<br> | 12/6/2012<br> | Xiuli<br> |- | [[1437 pT7A3-RNaseA|1437]]<br> | RNAse A<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1458 pT7A3-KOD|1458]]<br> | KOD<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1494 pT7A3-UDG|1494]]<br> | UDG<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1495-PT7A3-MMLV|1495]]<br> | MMLV<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1495-1496-1497-screening]]<br> | 12/20/2012<br> | yingying<br> |- | [[1496-pT7A3-DNase1|1496]]<br> | Dnase1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1495-1496-1497-screening]]<br> | 12/20/2012<br> | yingying<br> |- | [[1497-pT7A3-ToughTaq|1497]]<br> | ToughTaq<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 3ml, 37,0.5mM IPTG O/N<br> | Yes<br> | Yes<br> | [[1495-1496-1497-screening]]<br> | 12/20/2012<br> | yingying<br> |- | [[1498-pT7A3-TaqDNALigase|1498]]<br> | Tagligase<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | Yes<br> | Yes<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1505 pT7A3-T7-Endo1|1505]]<br> | T7-endo1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1508 pT7A3-DNA-Pol-1|1508]]<br> | DNA-pol-1<br> | pT7A3<br> | BL21(DE3)Star<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1437 1458 1494 1497 1498 1505 1508]]<br> | 12/31/2012<br> | yingying<br> |- | [[1510 pT7A3-MBP.Native-3C|1510]]<br> | MBP-native 3C<br> | pT7A3<br> | <br> | 5ml, 37,0.5mM IPTG O/N<br> | only MBP <br> | only MBP <br> | [[1465 1510 1511 1514 1517]] [[1510 1511]] <br> | 12/31/2012 1/3/2013<br> | yingying<br> |- | [[1511 pT7A3-MBP.Synthetic-3C|1511]]<br> | MBP-synthetic 3C<br> | pT7A3<br> | <br> | 5ml, 37,0.5mM IPTG O/N<br> | only MBP <br> | only MBP <br> | [[1465 1510 1511 1514 1517]] [[1510 1511]] | 12/31/2012 1/3/2013<br> | yingying<br> |- | [[1514 pT7A3-MBP-(3C)|1514]]<br> | MBP- 3C<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | No<br> | No<br> | [[1465 1510 1511 1514 1517]]<br> | 12/31/2012<br> | yingying<br> |- | [[1517 pT7A3-MBP-(3C)-sfGFP|1517]]<br> | MBP-sfGFP<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 37,0.5mM IPTG O/N<br> | Yes<br> | Yes<br> | [[1465 1510 1511 1514 1517]]<br> | 12/31/2012<br> | yingying<br> |- | [[1554-pT7A3-MBP-i3C|1554]]<br> | MBP-i3C<br> | pT7A3<br> | BL21DE3pRare2,BL21DE3pRare2pLysS<br> | 5ml, 25,0.3mM IPTG O/N<br> | only MBP <br> | only MBP <br> | [[1554 1613]]<br> | 2013/1/18<br> | yingying<br> |- | [[1613-pET28-cold-GST-3C|1613]]<br> | GST-3C<br> | pET28b<br> | BL21DE3pRare2,BL21DE3pRare2pLysS<br> | 5ml, 25,0.3mM IPTG O/N<br> | Yes<br> | Yes<br> | [[1554 1613]]<br> | 2013/1/18<br> | yingying<br> |- | [[1049 pT7A3-T4 RNA Ligase 1|1049 ]]<br> | T4_RNA_Ligase_1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml,<br> 0.2 mM IPTG RT o/n | Yes<br> | Yes<br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1123 pT7A3-MBP-(3C)-T4-RNA-Lig2|1123 ]]<br> | MBP-(3C)-T4-RNA-Lig2<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | Yes<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1501 pT7A3-Klenow|1501 ]]<br> | Klenow<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | no<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1503 pT7A3-T4 RNA Ligase 2 Truncated|1503 ]]<br> | T4_RNA_Ligase_2_Truncated<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1508 pT7A3-DNA-Pol-1|1508 ]]<br> | DNA-Pol-1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1512 pT7A3-Phi29DNAPol|1512 ]]<br> | Phi29DNAPol<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1513-pT7A3-polyAPol|1513]]<br> | polyAPol<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | no<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1537 pT7A3-Klenow3'-5'exo–|1537]]<br> | Klenow3'-5'exo–<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | no<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1557 pT7A3-MBP-(3c)-Lambda-Exo|1557 ]]<br> | MBP-(3c)-Lambda-Exo<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1572 pT7A3-MBP-(3c)-Endonuclease VIII|1572 ]]<br> | MBP-(3c)-Endonuclease_VIII<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]<br> | 2013/3/11<br> | Shanyi<br> |- | [[1509-pT7A3-hTdT|1509]]<br> | hTdT<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1533 pET28b-T7-DNA-Polymerase|1533]]<br> | T7-DNA-Polymerase<br> | pET28b<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1539-pET28b-T7-DNA-Pol-D28|1539]]<br> | T7-DNA-Pol-D28human ribonuclease/angiogenin inhibitor 1 | pET28b<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1569 pT7A3-MBB-(3C)-Exo-III|1569]]<br> | MBP-(3C)-Exo-III<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> |- | [[1581 pT7A3-T4RNA-Lig2-truncated-KQ|1581]]<br> | T4RNA-Lig2-truncated-KQ<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1591 pT7A3-MBP-(3c)-Ecoli-UDG|1591]]<br> | MBP-(3c)-Ecoli-UDG<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1617 pT7A3-MBP-(3c)-newPhi29|1617]]<br> | MBP-(3c)-newPhi29<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1570 pT7A3-MBP-(3C)-Exo-1|1570]]<br> | MBP-(3C)-Exo-1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1501 pT7A3-Klenow|1501]]<br> | Klenow<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.1 mM IPTG RT o/n<br> | ?<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1537 pT7A3-Klenow3'-5'exo–|1537]]<br> | Klenow3'-5'exo–<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.1 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1529 pT7A3-Ecoli RecA|1529]]<br> | Ecoli_RecA<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]<br> | 2013/3/20<br> | Shanyi<br> |- | [[1542-pT7A3-sso7d-Native-ToughTaq|1542]]<br> | Native-ToughTaq<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1542 1544 1545 1627 1679 1680]]<br> | 2013/3/28<br> | yingying<br> |- | [[1543-pT7A3-VVCap-S|1544]]<br> | VVCap-L<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1542 1544 1545 1627 1679 1680]]<br> | 2013/3/28<br> | yingying<br> |- | [[1545-pT7A3-VVCap|1545]]<br> | VVCap<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | stop inter<br> | <br> | [[1542 1544 1545 1627 1679 1680]]<br> | 2013/3/28<br> | yingying<br> |- | [[1627-pT7A3-SOD|1627]]<br> | SOD<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1542 1544 1545 1627 1679 1680]]<br> | 2013/3/28<br> | yingying<br> |- | [[1097-pET28b-T4-G32|1097]]<br> | T4-G32<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1138 p8H-MutM|1138]]<br> | MutM<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1458 pT7A3-KOD|1458]]<br> | KOD<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1494 pT7A3-UDG|1494]]<br> | UDG<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1505 pT7A3-T7-Endo1|1505]]<br> | T7-Endo1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1513-pT7A3-polyAPol|1513]]<br> | polyAPol<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1558 pT7A3-T7-Exo|1558]]<br> | T7-Exo<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1562 pT7A3-T4-Endonuclease-VII|1562]]<br> | T4-Endonuclease-VII<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1573-pT7A3-MBP-(3C)-RnaseH|1573]]<br> | MBP-(3C)-RnaseH<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1593 pT7A3-MBP-(3c)-E coli Endo IV|1593]]<br> | MBP-(3c)-E_coli_Endo_IV<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1654 pT7A3-T3 DNA ligase|1654]]<br> | T3_DNA_ligase<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]<br> | 2013/3/29<br> | Shanyi<br> |- | [[1567 pT7A3-T5-Exo|1567]]<br> | T5-Exo<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1696 1707 1567 1673 1697]]<br> | 2013/4/5<br> | Paul<br> |- | [[1614-pCold-TF-(3c)|1614]]<br> | TF<br> | pCold<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]<br> | 2013/3/30<br> | Paul<br> |- | [[1673-pET28b-NdeI|1673]]<br> | Nde1<br> | pET28b<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1696 1707 1567 1673 1697]]<br> | 2013/4/5<br> | Paul<br> |- | [[1677-pTCold-MBP-(3C)|1677]]<br> | MBP<br> | pTCold<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]<br> | 2013/3/30<br> | Paul<br> |- | [[1694-pT7A3-wtSM2|1692]]<br> | MBP<br> | pCold<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | <br> | <br> | <br> | <br> | Paul<br> |- | [[1694-pT7A3-wtSM2|1694]]<br> | wtSM2<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | <br> | <br> | <br> | 2013/3/30<br> | Paul<br> |- | [[1697-pCold-GST-(3c)-wt3C|1696]]<br> | MBP-(3C)-TEV<br> | pCold-<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]<br> | 2013/3/30<br> | Paul<br> |- | [[1697-pCold-GST-(3c)-wt3C|1697]]<br> | GST-(3c)-wt3C<br> | pCold-<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1696 1707 1567 1673 1697]]<br> | 2013/4/5<br> | Paul<br> |- | [[1707-pT7A3-MBP-(E3C)-p53wt|1707]]<br> | MBP-(E3C)-p53wt<br> | pT7A3-<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]<br> | 2013/3/30<br> | Paul<br> |- | [[623 pT7A3-8H-IL-4|623]]<br> | <br> pT7A3-8H-IL-4<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1248 pT7A3-DeepVent-Exo|1248]]<br> | DeepVent-Exo<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1285 pT7A3-Proteinase-K|1285]]<br> | Proteinase-K<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1313 pT7A3-RNAse 1|1313]]<br> | RNAse_1<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1314 pT7-RppH|1314]]<br> | RppH<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1317 pT7A3-RNH1|1317]]<br> | RNH1(human ribonuclease/angiogenin inhibitor 1)<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1494 pT7A3-UDG|1494]]<br> | UDG<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1536 pT7A3-RNH1|1536]]<br> | RNH1(Human RNase Inhibitor)<br> | pT7A3<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1705 pT7A3-ZB2-T4Lysozyme|1705]]<br> | ZB2-T4Lysozyme<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1706 pT7A3-BirA|1706]]<br> | BirA <br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1732 pT7A3-Ecoli-Ligase|1732]]<br> | Ecoli-Ligase<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1437 pT7A3-RNaseA|1437]]<br> | RNaseA<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YES<br> | <br> | [[1437 1568]]<br> | 2013/05/02<br> | Shanyi<br> |- | [[1568 pT7A3-Exo-VIII|1568]] <br> | MBP-Exo-VIII<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | YSE<br> | <br> | [[1437 1568]]<br> | <br> | <br> |- | 1711<br> | hINFA2<br> | pET28b<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1458 1654 1711 1712]]<br> | 2013/4/12<br> | yingying |- | 1712<br> | hINFA2<br> | pLambda<br> | BL21DE3pRare2pLysS<br> | 37 oc culture, 42 oC induction for 2h<br> | yes<br> | <br> | [[1458 1654 1711 1712]]<br> | 2013/4/12<br> | yingying<br> |- | [[626-pT7A3-Taq d289|626]]<br> | Taq d289<br> | pT7A3<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[626 1717 1719 1720]]<br> | 2013/5/2<br> | yingying<br> |- | [[1717-pT7A3-6H-(TEV)-MBP-(3C)|1717]]<br> | MBP<br> | pT7A3-MBP-(E3C)<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[626 1717 1719 1720]]<br> | 2013/5/2<br> | yingying<br> |- | [[1719-pT7A3-MBP-(E3C)-NRF2|1719]]<br> | NRF2<br> | pT7A3-MBP-(E3C)<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[626 1717 1719 1720]]<br> | 2013/5/2<br> | yingying<br> |- | [[1720-pT7A3-MBP-(E3C)-Keap1|1720]]<br> | Keap1<br> | pT7A3-MBP-(E3C)<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?<br> | <br> | [[626 1717 1719 1720]]<br> | 2013/5/2<br> | yingying<br> |- | [[1729-pCold-TF-(E3C)-3C|1729]]<br> | TF-(E3C)-3C<br> | pCold-TF-(E3C)-3C<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]<br> | 2013/5/21<br> | yingying<br> |- | [[1724-pT7A3-6His-sc-VVCap|1724]]<br> | sc-VVCap<br> | pT7A3-6His-sc-VVCap<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]<br> | 2013/5/21<br> | yingying<br> |- | [[1741-pIC-sfGFP|1741]]<br> | sfGFP<br> | pIC-sfGFP<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]<br> | 2013/5/21<br> | yingying<br> |- | [[1745-p8HIC-sfGFP|1745]]<br> | sfGFP<br> | p8HIC-sfGFP<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]<br> | 2013/5/21yingying<br> |- | [[643-pT7A3-Nhe1|643]]<br> | Nhe1<br> | pT7A3-Nhe1<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[645-pT7A3-Pst1|645]]<br> | Pst1<br> | pT7A3-Pst1<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[652-pT7A30-hIFNA2|652]]<br> | hIFNA2<br> | pT7A30-hIFNA2<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | weak<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[1736-pT7A3-His-NusA|1736]]<br> | His-NusA<br> | pT7A3-His-NusA<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[1764-pT7A3-T4-Pnk|1764]]<br> | T4-Pnk<br> | pT7A3-T4-Pnk<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[1771-pT7A3-MBP-(3C)-PCK1|1771]]<br> | PCK1<br> | pT7A3-MBP-(3C)-PCK1<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[1721-pT7A3-MBP-(E3C)-SOD|1721]]<br> | MBP-(E3C)-SOD<br> | pT7A3-MBP-(E3C)-SOD<br> | Nico21(DE3)plysN<br> | 5ml, 0.2 mM IPTG RT o/n<br> | only MBP<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[1731-pT7A3-TurboEnterokinase|1731]]<br> | TurboEnterokinase<br> | pT7A3-TurboEnterokinase<br> | BL21DE3pRare2pLysS<br> | 5ml, 0.2 mM IPTG RT o/n<br> | ?weak<br> | <br> | [[643 645 652 1721 1731 1736 1757 1764 1771]]<br> | 2013/7/9yingying<br> |- | [[631-pT7A3-IL-22|631]]<br> | IL-22<br> | pT7A3-IL-22<br> | NiCO21(DE3)pLysN<br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | NO<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1497-pT7A3-ToughTaq|1497]]<br> | ToughTaq<br> | pT7A3-ToughTaq<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,37C 0.2 mM IPTG RT o/n<br> | weak<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1542-pT7A3-sso7d-Native-ToughTaq|1542]]<br> | sso7d-Native-ToughTaq<br> | pT7A3-sso7d-Native-ToughTaq<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,37C 0.2 mM IPTG RT o/n<br> | weak<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1719-pT7A3-MBP-(E3C)-NRF2|1719]]<br> | MBP-(E3C)-NRF2<br> | pT7A3-MBP-(E3C)-NRF2<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1748-pCold-8His-MBP-NRF2-6His|1748]]<br> | -MBP-NRF2<br> | pCold-8His-MBP-NRF2-6His<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1765-pT7A3-T4DNAPol|1765]]<br> | T4DNAPol<br> | pT7A3-T4DNAPol<br> | Nico21(DE3)plysN<br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1780-pT7A3-T7-RNAPol-Y639F|1780]]<br> | T7-RNAPol-Y639F<br> | pT7A3-T7-RNAPol-Y639F<br> | Nico21(DE3)plysN<br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | no<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1781-pTCold-MBP-(3C-TEV)-PKARIIb|1781]]<br> | MBP-(3C-TEV)-PKARIIb<br> | pTCold-MBP-(3C-TEV)-PKARIIb<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1782-pT7A3-Tth-Ligase|1782]]<br> | Tth-Ligase <br> | pT7A3-Tth-Ligase <br> | Nico21(DE3)plysN<br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | yes<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | [[1797-pCold-6His-3C-MBP|1797]]<br> | 3C-MBP<br> | pCold-6His-3C-MBP<br> | BL21(DE3)Star, pRare2-pLysS <br> | 5ml,25C 0.2 mM IPTG RT o/n<br> | MBP and 3C seperated<br> | <br> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]<br> | 2013/7/30 yingying<br> |- | <br> | <br> | <br> | <br> | <br> | <br> | <br> | <br> | <br> |} <br> [[Category:Protein_Expression]]
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