Protein Expression Clone Screening
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E coli Expression Screening 2012
[edit]sfGFP
| Clone ID | Protein Name | Vector | Host | Condition | Expression Level | Solubility | Gel Image (Link) | Date | By |
| 360 |
MBP |
pDB.His |
Nico21(DE3)plysN |
3ml,37,0.2mMIPTG,5h |
high |
yes |
MBP,MMLV,UDG Protein Screening |
11/7/2012 |
yingying |
| 1428 |
MBP |
pT7 |
Nico21(DE3)plysN |
2ml,37,0.2mMIPTG,5h |
no |
no |
PT7-MBP-1428,pT7A3-MBP-1429,Nov,2,2012 |
11/2/2012 |
yingying |
| 1429 |
MBP |
pT7A3 |
Nico21(DE3)plysN |
3ml,37,0.2mMIPTG,5h |
high |
yes |
11/2/2012 11/7/2012 |
yingying | |
| 501 |
MMLV |
PET30b |
Nico21(DE3)plysN |
3ml,37,0.2mMIPTG,5h |
no | no |
MBP,MMLV,UDG Protein Screening |
11/7/2012 |
yingying |
| 1034 |
UDG |
PET8H |
Nico21(DE3)plysN |
3ml,37,0.2mMIPTG,5h |
yes |
no |
MBP,MMLV,UDG Protein Screening |
11/7/2012 |
yingying |
| 1375 |
MBP-3C |
pDB-His |
Nico21(DE3)plysN |
1L,37, 0.3mMIPTG,4h |
yes,only MBP, not 3C |
Yes |
PDB.His.MBP-3C |
10/15/2012 |
Xiuli |
| 1308 |
CRE |
pET6H |
Nico21(DE3)plysN |
2ml,37,0.5mMIPTG,4h |
Yes, but leaky, expressed in uninduced |
Yes |
PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre |
Xiuli | |
| 1311 |
CRE |
pET6H |
Nico21(DE3)plysN |
2ml,37,0.5mMIPTG,4h |
High |
Yes |
PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre |
Xiuli | |
| 1307 |
CRE |
pET6H |
Nico21(DE3)plysN |
2ml,37,0.5mMIPTG,4h |
Faint, need recheck |
Yes |
1307-1309-1310 screening |
11/7/2-12 |
Xiuli |
| 1310 |
CRE |
pET6H |
Nico21(DE3)plysN |
2ml,37,0.5mMIPTG,4h |
High |
Yes |
1307-1309-1310 screening |
11/7/2012 |
Xiuli |
| 1309 |
CRE |
pET6H |
Nico21(DE3)plysN |
2ml,37,0.5mMIPTG,4h |
High |
Yes |
1307-1309-1310 screening |
11/7/2012 |
Xiuli |
| 1143 |
Exo-1 |
pET8H |
Nico21(DE3)plysN |
3 ml, 37, 0.5mM IPTG, O/N |
no |
no |
1143-1151-1186-screening |
11/16/2012 |
Xiuli |
| 1151 |
sso7d ToughTag, |
pET30b |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
no |
no |
1143-1151-1186-screening |
11/16/2012 |
Xiuli |
| 1155 |
Ara-S1 |
pET28b |
Nico21(DE3)plysN |
3 ml,37,0.5mM IPTG O/N |
no |
no |
1143-1151-1186-screening |
11/16/2012 |
Xiuli |
| 1156 |
Vent Exo |
p8H |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
Yes |
Yes |
1143-1151-1186-screening |
11/16/2012 | |
| 1186 |
MBP-Xis |
pDB.Xis |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG, O/N |
Yes |
Yes |
1143-1151-1186-screening |
11/16/2012 |
Xiuli |
| 1140 |
polyAPol |
pTXB1 |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG 4 hs |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1325 |
MBP-gpI3L |
pDBHis |
Nico21(DE3)plysN |
3 ml,37,0.5mM IPTG 4 hsYes |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1225 |
T7-Endo1 |
pET28b |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1206 |
recT |
pET28b |
Nico21(DE3)plysN |
3 ml,37,0.5mM IPTG, O/N |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1203 |
scIHF2 |
pET42b |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1245 |
PfuMBP.scIHF2 |
pDBHIS |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1249 |
Taq_d289 |
pET |
Nico21(DE3)plysN |
3 ml, 37,0.5mM IPTG O/N |
No |
no |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli |
| 1464 |
T7 ligase |
pT7A3 |
Nico21(DE3)plysN |
Yes |
Yes |
1140 1325 1225 1206 1203 1245 1249 1464 |
11/30/2012 |
Xiuli | |
| 1137 |
Exo-VIII |
p8H |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG 4 hrs |
No |
No |
1137-1121-1291-1252-1206 |
12/6/2012 |
Xiuli |
| 1121 |
Exo-1 |
pET28b |
Nico21(DE3)plysN |
3ml,37,0.5mM IPTG 4hrs |
No |
No |
1137-1121-1291-1252-1206 | 12/6/2012 |
Xiuli |
| 1291 |
TAT-NLS-sso7d |
pET30b |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG 4hrs |
Yes |
Yes |
1137-1121-1291-1252-1206 |
12/6/2012 |
Xiuli |
| 1252 |
hLysozyme |
pTXB1 |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG 4 hrs |
Yes |
No |
1137-1121-1291-1252-1206 |
12/6/2012 |
Xiuli |
| 1206 |
recT |
pET28b |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG 4 hrs |
No |
No |
1137-1121-1291-1252-1206 |
12/6/2012 |
Xiuli |
| 1437 |
RNAse A |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1458 |
KOD |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1494 |
UDG |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1495 |
MMLV |
pT7A3 |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG
O/N |
No |
No |
1495-1496-1497-screening |
12/20/2012 |
yingying |
| 1496 |
Dnase1 |
pT7A3 |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG
O/N |
No |
No |
1495-1496-1497-screening |
12/20/2012 |
yingying |
| 1497 |
ToughTaq |
pT7A3 |
Nico21(DE3)plysN |
3ml, 37,0.5mM IPTG
O/N |
Yes |
Yes |
1495-1496-1497-screening |
12/20/2012 |
yingying |
| 1498 |
Tagligase |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
Yes |
Yes |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1505 |
T7-endo1 |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1508 |
DNA-pol-1 |
pT7A3 |
BL21(DE3)Star |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1437 1458 1494 1497 1498 1505 1508 |
12/31/2012 |
yingying |
| 1510 |
MBP-native 3C |
pT7A3 |
5ml, 37,0.5mM IPTG
O/N |
only MBP |
only MBP |
|
12/31/2012 1/3/2013 |
yingying | |
| 1511 |
MBP-synthetic 3C |
pT7A3 |
5ml, 37,0.5mM IPTG
O/N |
only MBP |
only MBP |
12/31/2012 1/3/2013 |
yingying | ||
| 1514 |
MBP- 3C |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
No |
No |
1465 1510 1511 1514 1517 |
12/31/2012 |
yingying |
| 1517 |
MBP-sfGFP |
pT7A3 |
Nico21(DE3)plysN |
5ml, 37,0.5mM IPTG
O/N |
Yes |
Yes |
1465 1510 1511 1514 1517 |
12/31/2012 |
yingying |
| 1554 |
MBP-i3C |
pT7A3 |
BL21DE3pRare2,BL21DE3pRare2pLysS |
5ml, 25,0.3mM IPTG
O/N |
only MBP |
only MBP |
1554 1613 |
2013/1/18 |
yingying |
| 1613 |
GST-3C |
pET28b |
BL21DE3pRare2,BL21DE3pRare2pLysS |
5ml, 25,0.3mM IPTG
O/N |
Yes |
Yes |
1554 1613 |
2013/1/18 |
yingying |
| 1049 |
T4_RNA_Ligase_1 |
pT7A3 |
Nico21(DE3)plysN |
5ml, 0.2 mM IPTG RT o/n |
Yes |
Yes |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi |
| 1123 |
MBP-(3C)-T4-RNA-Lig2 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
Yes |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1501 |
Klenow |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
no |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1503 |
T4_RNA_Ligase_2_Truncated |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1508 |
DNA-Pol-1 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1512 |
Phi29DNAPol |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1513 |
polyAPol |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
no |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1537 |
Klenow3'-5'exo– |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
no |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1557 |
MBP-(3c)-Lambda-Exo |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
? |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1572 |
MBP-(3c)-Endonuclease_VIII |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1049 1123 1501 1503 1508 1512 1513 1537 1557 1572 |
2013/3/11 |
Shanyi | |
| 1509 |
hTdT |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1533 |
T7-DNA-Polymerase |
pET28b |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1539 |
T7-DNA-Pol-D28human ribonuclease/angiogenin inhibitor 1 | pET28b |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1569 |
MBP-(3C)-Exo-III |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 | ||
| 1581 |
T4RNA-Lig2-truncated-KQ |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1591 |
MBP-(3c)-Ecoli-UDG |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1617 |
MBP-(3c)-newPhi29 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
? |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1570 |
MBP-(3C)-Exo-1 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1501 |
Klenow |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.1 mM IPTG RT o/n |
? |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1537 |
Klenow3'-5'exo– |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.1 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1529 |
Ecoli_RecA |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529 |
2013/3/20 |
Shanyi | |
| 1542 |
Native-ToughTaq |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1542 1544 1545 1627 1679 1680 |
2013/3/28 |
yingying | |
| 1544 |
VVCap-L |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1542 1544 1545 1627 1679 1680 |
2013/3/28 |
yingying | |
| 1545 |
VVCap |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
stop inter |
1542 1544 1545 1627 1679 1680 |
2013/3/28 |
yingying | |
| 1627 |
SOD |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1542 1544 1545 1627 1679 1680 |
2013/3/28 |
yingying | |
| 1097 |
T4-G32 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1138 |
MutM |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1458 |
KOD |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1494 |
UDG |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1505 |
T7-Endo1 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1513 |
polyAPol |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1558 |
T7-Exo |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1562 |
T4-Endonuclease-VII |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1573 |
MBP-(3C)-RnaseH |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1593 |
MBP-(3c)-E_coli_Endo_IV |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1654 |
T3_DNA_ligase |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654 |
2013/3/29 |
Shanyi | |
| 1567 |
T5-Exo |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1696 1707 1567 1673 1697 |
2013/4/5 |
Paul | |
| 1614 |
TF |
pCold |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1567 1614 1673 1677 1692 1694 1696 1697 1707 1711 |
2013/3/30 |
Paul | |
| 1673 |
Nde1 |
pET28b |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1696 1707 1567 1673 1697 |
2013/4/5 |
Paul | |
| 1677 |
MBP |
pTCold |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1567 1614 1673 1677 1692 1694 1696 1697 1707 1711 |
2013/3/30 |
Paul | |
| 1692 |
MBP |
pCold |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
Paul | ||||
| 1694 |
wtSM2 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
2013/3/30 |
Paul | |||
| 1696 |
MBP-(3C)-TEV |
pCold- |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1567 1614 1673 1677 1692 1694 1696 1697 1707 1711 |
2013/3/30 |
Paul | |
| 1697 |
GST-(3c)-wt3C |
pCold- |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1696 1707 1567 1673 1697 |
2013/4/5 |
Paul | |
| 1707 |
MBP-(E3C)-p53wt |
pT7A3- |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1567 1614 1673 1677 1692 1694 1696 1697 1707 1711 |
2013/3/30 |
Paul | |
| 623 |
pT7A3-8H-IL-4 |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
? |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1248 |
DeepVent-Exo |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1285 |
Proteinase-K |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1313 |
RNAse_1 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1314 |
RppH |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1317 |
RNH1(human ribonuclease/angiogenin inhibitor 1) |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
NO |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1494 |
UDG |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
? |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1536 |
RNH1(Human RNase Inhibitor) |
pT7A3 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
NO |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1705 |
ZB2-T4Lysozyme |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
NO |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1706 |
BirA |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1732 |
Ecoli-Ligase |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732 |
2013/05/02 |
Shanyi | |
| 1437 |
RNaseA |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YES |
1437 1568 |
2013/05/02 |
Shanyi | |
|
|
MBP-Exo-VIII |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
YSE |
1437 1568 |
|||
| 1711 |
hINFA2 |
pET28b |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1458 1654 1711 1712 |
2013/4/12 |
yingying | |
| 1712 |
hINFA2 |
pLambda |
BL21DE3pRare2pLysS |
37 oc culture, 42 oC induction for 2h |
yes |
1458 1654 1711 1712 |
2013/4/12 |
yingying | |
| 626 |
Taq d289 |
pT7A3 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
? |
626 1717 1719 1720 |
2013/5/2 |
yingying | |
| MBP |
pT7A3-MBP-(E3C) |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
626 1717 1719 1720 |
2013/5/2 |
yingying | ||
| 1719 |
NRF2 |
pT7A3-MBP-(E3C) |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
? |
626 1717 1719 1720 |
2013/5/2 |
yingying | |
| 1720 |
Keap1 |
pT7A3-MBP-(E3C) |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
? |
626 1717 1719 1720 |
2013/5/2 |
yingying | |
| 1729 |
TF-(E3C)-3C |
pCold-TF-(E3C)-3C |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
1724 1729 1731 1741 1742 1743 1744 1745 1748 1749 |
2013/5/21 |
yingying | |
| 1724 |
sc-VVCap |
pT7A3-6His-sc-VVCap |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1724 1729 1731 1741 1742 1743 1744 1745 1748 1749 |
2013/5/21 |
yingying | |
| 1741 |
sfGFP |
pIC-sfGFP |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1724 1729 1731 1741 1742 1743 1744 1745 1748 1749 |
2013/5/21 |
yingying | |
| 1745 |
sfGFP |
p8HIC-sfGFP |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
1724 1729 1731 1741 1742 1743 1744 1745 1748 1749 |
2013/5/21yingying | ||
| 643 |
Nhe1 |
pT7A3-Nhe1 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 645 |
Pst1 |
pT7A3-Pst1 |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 652 |
hIFNA2 |
pT7A30-hIFNA2 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
weak |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 1736 |
His-NusA |
pT7A3-His-NusA |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 1764 |
T4-Pnk |
pT7A3-T4-Pnk |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
yes |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 1771 |
PCK1 |
pT7A3-MBP-(3C)-PCK1 |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
yes |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 1721 |
MBP-(E3C)-SOD |
pT7A3-MBP-(E3C)-SOD |
Nico21(DE3)plysN |
5ml,
0.2 mM IPTG RT o/n |
only MBP |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 1731 |
TurboEnterokinase |
pT7A3-TurboEnterokinase |
BL21DE3pRare2pLysS |
5ml,
0.2 mM IPTG RT o/n |
?weak |
643 645 652 1721 1731 1736 1757 1764 1771 |
2013/7/9yingying | ||
| 631 |
IL-22 |
pT7A3-IL-22 |
NiCO21(DE3)pLysN |
5ml,25C
0.2 mM IPTG RT o/n |
NO |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30 yingying | ||
| 1497 |
ToughTaq |
pT7A3-ToughTaq |
BL21(DE3)Star, pRare2-pLysS |
5ml,37C
0.2 mM IPTG RT o/n |
weak |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1542 |
sso7d-Native-ToughTaq |
pT7A3-sso7d-Native-ToughTaq |
BL21(DE3)Star, pRare2-pLysS |
5ml,37C
0.2 mM IPTG RT o/n |
weak |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1719 |
MBP-(E3C)-NRF2 |
pT7A3-MBP-(E3C)-NRF2 |
BL21(DE3)Star, pRare2-pLysS |
5ml,25C
0.2 mM IPTG RT o/n |
yes |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1748 |
-MBP-NRF2 |
pCold-8His-MBP-NRF2-6His |
BL21(DE3)Star, pRare2-pLysS |
5ml,25C
0.2 mM IPTG RT o/n |
yes |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1765 |
T4DNAPol |
pT7A3-T4DNAPol |
Nico21(DE3)plysN |
5ml,25C
0.2 mM IPTG RT o/n |
yes |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1780 |
T7-RNAPol-Y639F |
pT7A3-T7-RNAPol-Y639F |
Nico21(DE3)plysN |
5ml,25C
0.2 mM IPTG RT o/n |
no |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1781 |
MBP-(3C-TEV)-PKARIIb |
pTCold-MBP-(3C-TEV)-PKARIIb |
BL21(DE3)Star, pRare2-pLysS |
5ml,25C
0.2 mM IPTG RT o/n |
yes |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1782 |
Tth-Ligase |
pT7A3-Tth-Ligase |
Nico21(DE3)plysN |
5ml,25C
0.2 mM IPTG RT o/n |
yes |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||
| 1797 |
3C-MBP |
pCold-6His-3C-MBP |
BL21(DE3)Star, pRare2-pLysS |
5ml,25C
0.2 mM IPTG RT o/n |
MBP and 3C seperated |
631_1497_1542_1719_1748_1765_1780_1781_1782_1797 |
2013/7/30
yingying | ||