1458 1654 1711 1712
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Data:2013-4-17
Exprimenter: SHANYI
| lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 | ||||
| clone ID | 1458 |
Marker |
1654 |
1711 |
1712 | |||||||||||
| name |
pT7A3-KOD |
pT7A3-T3 DNA ligase |
pET28b-hINFA2 |
pLambda-hINFA2 | ||||||||||||
| host |
Nico21DE3plysN |
Nico21DE3plysN |
BL21(DE3)pRare2pLysS |
BL21(DE3)pRare2pLysS | ||||||||||||
| induction |
0.2 mM IPTG 25oC o/n |
0.2 mM IPTG 25oC o/n |
0.2 mM IPTG 25oC o/n |
37 oc culture,OD=1.0, 42 oC induction 2h | ||||||||||||
| purification |
10ml HisTrap HP(2nd time) |
Bio-Sil TSK 250 |
Ni beads |
Ni beads | ||||||||||||
| MW |
7.53 / 90022.42 |
5.14 / 39350.70 |
21.6KD/5.95 |
21.6KD/5.95 | ||||||||||||