1536 pT7A3-RNH1
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Cloning of 1536 pT7A3-RNH1
[edit]Purpose: Cloning of 1536 pT7A3-RNH1
Date: 2013-2-4---
Notebook:0
Page:
Clone ID:1536
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR using
hRNH1-Nde1-F
hRNH1-Xho1-R
template 1531 pENTR223-RNH1
CUT OUT THE FIRST BAND (FROM UP TO DOWN) DIGEST WITH Nde1 + Xho1
| band 2 |
band 3,4,5 | |
| 1279 |
PCR | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-
Make miniprep and digest withNde1 + Xho1
Result intepretation/Summary
[edit]- <br>
Conclusion
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