1510 1511

Data:2013-1-1---2013-1-4

Exprimenter: yingying

5 ml sample each , 1510,1511  induce with 0.2mM IPTG at  37 oC 0/N overnight

1510	pT7A3-MBP.Native-3C
1511	pT7A3-MBP.Synthetic-3C

	1	2	3
Clone ID	1510	1510	1511
competent cell	Rosetta2 plysS	BL21 Star	Nico21 DE3 plysN

 conclusion : only MBP express ,no 3C

2013/1/7--2013/1/9

0.1 mM IPTG

	1	2	3	4	5	6
Clone ID	1510	1510	1511	1510	1510	1511
Competent cell	BL21 Star	Rosetta2 plysS	Nico21DE3 plysN	BL21 Star	Rosetta2 plysS	Nico21DE3 plysN
Temp	4 C overnight			18.5 C overnight

2013-1-13---2013-1-15

	1	2	3	4	5	6
clone ID	1510				1510	1511
IPTG mM	0.01	0.02	0.05	0.1	0.1	0.1
competent cell	BL21 Star				Rosetta2 DE3 plysS	Nico21DE3 plysN
Temp	25C overnight