1285 pT7A3-Proteinase-K
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Cloning of 1285 pT7A3-Proteinase-K
[edit]Purpose: Cloning of1285 pT7A3-Proteinase-K
Date: 2013-3-
Notebook:
Page:
Clone ID:1285
Experimenters: Shanyi
Results
[edit]cloning
[edit]Date:2013-3-
Gene synthesis of two fragments and join them by PCR
digest with Nde1 + Xho1, clone into Nde1 + Xho1 digested 1279 pT7A3
then use Fragment1,2, as template
digest with Nde1+ Xho1
Colony Screening
[edit]Date: 2013
