1505 pT7A3-T7-Endo1

Cloning of 1505 pT7A3-T7-Endo1

Purpose: Cloning of 1505 pT7A3-T7-Endo1

Date: Dec , 2012

Notebook: 

Page:

Clone ID: 1505

Experimenters: Shanyi

Results

Cloning

subclone T7 Endonuclease I from 1225 pET28b-T7-Endo1 to 1279 pT7A3 using Nde1 + Xho1

=== Colony screen ===Idigest_for_1732.JPG‎

wrong size

Cloning

subclone T7 Endonuclease I from 1225 pET28b-T7-Endo1 to 1279 pT7A3 using Nde1 + Xho1

Colony screen

Protein Expression

2013/3/29
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
M	PageRuler Plus Prestained protein ladder
1	pT7A3-T4-G32	T4-G32	1097	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	4.82 / 33506.43
2	pT7A3-MBP-(3c)-MutM	MBP-(3c)-MutM	1138	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.68 / 30290.07+43.6
3	pT7A3-KOD	KOD	1458	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.53 / 90022.42
4	pT7A3-UDG	UDG	1494	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.45 / 27174.73
5	pT7A3-T7-Endo1	T7-Endo1	1505	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	9.54 / 17171.84
6	pT7A3-polyAPol	polyAPol	1513	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	9.67 / 53870.91
7	pT7A3-T7-Exo	T7-Exo	1558	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	4.93 / 34502.20
8	pT7A3-MBP-(3C)-Exo-1	MBP-(3C)-Exo-1	1562	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.80 / 18143.72
9	pT7A3-MBP-(3C)-RnaseH	MBP-(3C)-RnaseH	1573	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.43 / 17596.99 +43.6
10	pT7A3-MBP-(3c)-E_coli_Endo IV	MBP-(3c)-E_coli_Endo IV	1593	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	5.43 / 31479.53 +43.6
11	pT7A3-T3 DNA ligase	T3 DNA ligase	1654	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.14 / 39350.70