1554 1613

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Data:2013-1-15---2013-1-18

Exprimenter: yingying

5 ml sample each , 1554,1613  induce with 0.3mM IPTG at  25oC 0/N overnight

1554 pT7A3-MBP-i3C
1613 pET28-cold-GST-3C

1
 2
 3
 4
clone ID
                  1554
                       1613
competent cell
 BL21DE3pRare2
 BL21DE3pRare2pLysS
 BL21DE3pRare2
 BL21DE3pRare2pLysS







 conclusion : 1613,pET28-cold-GST-3C has expression (lane3,4) 47KD; 1554 expresses MBP


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