1429-pT7A3-MBP
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Cloning of 1429-pT7A3-MBP
[edit]Purpose: Cloning of 1429-pT7A3-MBP
Date: 2012-10-18---2012-10-20
Notebook:0
Page:
Clone ID:1429
Experimenters: yingying
Results
[edit]subcloning
[edit]
digest 1279 pT7A3 (v)with Xba1 + EcoR1---> vector
digest pDB.His.MBP or pDB.His.MBP.amp(lane3,4,5) with Xba1 + EcoR1--->insert
ligate and transform
Colony Screening
[edit]Date: 2012-10-20
Make miniprep and digest with Xba1 + EcoR1
clone1,2,3,4,5 right
Result intepretation/Summary
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Conclusion
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