1568 pT7A3-Exo-VIII
Cloning of 1568 pT7A3-Exo-VIII
[edit]Purpose: Cloning of 1568 pT7A3-Exo-VIII
Date: December 31, 2012
Notebook:
Page:
Clone ID: 1568
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: December 31, 2012
Subclone from 1137 (insert lane one) p8H-Exo-VIII using Nde1 + Xho1 and vector 1278 (pT7A3) lane 2
Cut out fragments, ligate together and transform
Date: Jan 3, 2012
Subclone from 1137 (insert lane 5) p8H-Exo-VIII using Nde1 + Xho1 and vector 1514 (pT7A3-MBP-(3C)) lane 6
Cut out fragments, ligate together and transform
Screening
[edit]Date: Jan 2, 2013
Digested MiniPrep with RE Nde1 and Xho1
See lanes 1-4 (samples 1-4)
Insert wrong size
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1568 pT7A3-MBP-(3C)-Exo-VIII
[edit]Purpose: Cloning of 1568 pT7A3-MBP-(3C)-Exo-VIII
Date: Jan, 2013
Notebook:
Page:
Clone ID: 1568
Experimenters: Shanyi
Results
[edit]Cloning
[edit]Subclone from 1137 (insert lane 5) p8H-Exo-VIII using Nde1 + Xho1 and vector 1514 (pT7A3-MBP-(3C)) lane 6
Cut out fragments, ligate together and transform
Screening
[edit]Date: Jan 2, 2013
Digested MiniPrep with RE Nde1 and Xho1
