1140 1325 1225 1206 1203 1245 1249 1464
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Protein Expression Screeening
Date:
Experimentor: Xiuli
Description:
Clones:
| 1140 |
pTXB1-polyAPol, E coli E coli poly(A) polymerase I |
| 1325 |
pDBHisMBP-gpI3L, vaccinia virus I3L gene |
| 1225 |
pET28b-T7-Endo1, T7 endonuclease 1 |
| 1206 |
pET28b-recT |
| 1203 |
pET42b-scIHF2 |
| 1245 |
pDB.His.PfuMBP.scIHF2 |
| 1249 |
pET-Taq_d289 |
| 1464 |
pT7A3-T7 ligase |
Gel 1 (supernant)
| M |
1325 |
1203 |
1245 |
1464 |
||||||
| pDBHisMBP-gpI3L |
pET42b-scIHF2 |
pDB.His.PfuMBP.scIHF2 |
pT7A3-T7 ligase |
MBP | ||||||
| Lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
| Sample |
C |
I |
C |
I |
C |
I |
I |
Pellet |
||
Gel 2 (Supernant)
pET28b-recT
| M |
1140 |
1225 |
1206 |
1249 |
1140 |
|||||||
| pTXB1-polyAPol |
pET28b-T7-Endo1 |
pET28b-recT |
pET-Taq_d289 |
pTXB1-polyAPol |
MBP | |||||||
| Lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| Sample |
C |
I |
C |
I |
C |
I |
C |
I |
C |
I |
||
Gel-3 (pellet)
pET28b-recT
| M |
1140 |
1225 |
1206 |
1249 |
1249-2 |
|||||||
| pTXB1-polyAPol |
pET28b-T7-Endo1 |
pET28b-recT |
pET-Taq_d289 |
pET-Taq_d289 |
MBP | |||||||
| Lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 | |
| Sample |
C |
I |
C |
I |
C |
I |
C |
I |
C |
I |
||
Gel 4 (pellet)
| M |
1325 |
1203 |
1245 |
1140-2 |
||||||
| pDBHisMBP-gpI3L |
pET42b-scIHF2 |
pDB.His.PfuMBP.scIHF2 |
pTXB1-polyAPol |
MBP | ||||||
| Lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
10 | |
| Sample |
C |
I |
C |
I |
C |
I |
C |
I |
||
