1782-pT7A3-Tth-Ligase
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Cloning of 1782-pT7A3-Tth-Ligase
[edit]Purpose: Cloning of 1782-pT7A3-Tth-Ligase
Date: 2013-7-12---2013-7-15
Notebook:0
Page:
Clone ID:1782
Experimenters: yingying
Results
[edit]cloning
[edit]
subclone from 1022pET28b-Tth-Ligase to 1279 pT7A3 using Nde1 + EcoR1
lane1:1022pET28b-Tth-Ligase
lane2:1279 pT7A3
Colony Screening
[edit]Date: 2013-7-15
Make miniprep and digest with Nde1 + EcoR1
clone1 for stock
need to do digestion again to confirm
2013-7-26
digest with BamH1+EcoR1, ~1000kb size
Result intepretation/Summary
[edit]Conclusion
[edit]