1745-p8HIC-sfGFP

Cloning of 1745-p8HIC-sfGFP

Purpose: Cloning of 1745-p8HIC-sfGFP

Date: 2013-5-8---2013-5-16

Notebook:0

Page:

Clone ID:1745

Experimenters: yingying

Results

cloning

Colony Screening

Date: 2013-5-16

Make miniprep and digest with Nhe1 + Xho1

Result intepretation/Summary

== Conclusion ==

2013-5-21

Ni beads

lane	clone ID	host	induction	purification	MW
1	1694,pT7A3-wtSM2	BL21(DE3)pRare2pLysS	0.2 mM IPTG 25 oC o/n	CBD beads, wrong buffer,need to do again	33KD
2	1724,pT7A3-6His-sc-VVCap	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	70KD
3	1729,pCold-TF-(E3C)-3C	BL21(DE3)pRare2pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	51KD+21KD
4	1731,pT7A3-TurboEnterokinase	BL21(DE3)pRare2pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	27KD
5	1741,pIC-sfGFP	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	no his tag,supernatant run gel	31KD
6	1742,p8H-ELK-IN	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	15KD
7	1743,pIC	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	4KD
8	1744,p8HIC	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	5KD
9	1745,p8HIC-sfGFP	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads,lysis supernatant is green	15KD
10	1748,pCold-8His-MBP-NRF2-6His	BL21(DE3)pRare2pLysS	0.2 mM IPTG 25 oC o/n	Ni beads
11	1749,p7H-ELK-IN	NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	15KD