1797-pCold-6His-3C-MBP
Jump to navigation
Jump to search
Cloning of 1797-pCold-6His-3C-MBP
[edit]Purpose: Cloning of 1797-pCold-6His-3C-MBP
Date: 2013-7-22---2013-7-24
Notebook:006026
Page:
Clone ID:1797
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR1 3C from 1420-pDB.His.MBP.3C
new-3C-1
HRV14-3C-MBP-R
PCR2 MBP from 1281 pMAL-p5E as template
HRV14-3C-MBP-F
MBP-Sal1-stop-R2
Join two fragments using
new-3C-1
MBP-Sal1-stop-R2
| lane |
1 |
2 |
| PCR product |
v19 pET28-cold | |
| enzymes |
Nde1 + Sal1 |
Nde1 + Xho1 |
Colony Screening
[edit]Date: 2013-7-24
Make miniprep and digest Nde1+Kpn1
