1567 1614 1673 1677 1692 1694 1696 1697 1707 1711

2013/3/30

Ni beads

lane	name	clone ID	host	induction	purification		MW
1	pT7A3-T5-Exo	1567	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		21KD/5.95
2	pCold-TF-(3c)	1614	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	55.9KD/5.19
3	pET28b-Nde1	1673	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		44KD/8.41
4	pTCold-MBP-(3C)	1677	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		44KD
5	pCold-C(3C)-MBP-6H	1692	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		46KD/5.77
6	pT7A3-wtSM2	1694	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads, should use CBD beads		33KD/6.30
7	pCold-MBP-(3C)-TEV	1696	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		72KD/6.04
8	pCold-GST-(3c)-wt3C	1697	4 ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads		21KD/8.47
9	pT7A3-MBP-(E3C)-p53wt	1707	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads		88KD/5.76
10	pET28b-hINFA2	1711	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads		21KD/5.95