1437 1458 1494 1497 1498 1505 1508

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Data:2012-12-29---2012-12-31

Exprimenter: yingying

3 ml sample each , 1437,1458,1494,1497,1505,1508  induce with 0.5mM IPTG at  37 oC 0/N overnight

1437 pT7A3-RNaseA
1458 pT7A3-KOD
1494 pT7A3-UDG
1497
 pT7A3-ToughTaq
1498
 pT7A3-TaqDNALigase
1505
 pT7A3-T7-Endo1
1508
 pT7A3-DNA-Pol-1



 1
2
 3
 4
 5
 6
 7
 8
 9
Clone ID
 1437
 1458
 1494
 1497
 1498
 1505
 1508
 1429
 1429control
competent cell
                   Nico21 DE3 plysN




 conclusion : 1498 pT7A3-TaqDNALigase has low expression (lane5) 76KD








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