623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732

Protein Expression

2013/5/2
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pT7A3-8H-IL-4	IL-4	623	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTGRT o/n	Ni beads	9.13/17492.34
2	pT7A3-DeepVent-Exo	DeepVent-Exo	1248	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	4.73 / 23943.39
3	pT7A3-Proteinase-K	Proteinase-K	1285	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.71 / 41026.85
M	PageRuler Plus Prestained protein ladder
4	pT7A3-RNAse 1	Ecoli RNAse 1	1313	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.64 /29617.59
5	pT7A3-RppH	RNA pyrophosphohydrolase	1314	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	10.36/ 20794.84
6	pT7A3-RNH1	human ribonuclease/angiogenin inhibitor 1	1317	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	4.71 / 49954.42
7	pT7A3-UDG	UDG	1494	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.45 / 27174.73
8	pT7A3-RNH1	Human RNase Inhibitor	1536	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	4.63 / 51409.96
9	pT7A3-ZB2-T4Lysozyme	ZB2-T4Lysozyme	1705	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	9.79 / 25625.35
10	pT7A3-BirA	Biotin Protein Ligase	1706	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	7.76 / 35312.00
11	pT7A3-Ecoli-Ligase	Ecoli-Ligase	1732	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.39 / 73606.07