1375-pDB.His.MBP.3C

Cloning of 1375 pDB.His.MBP.3C

Purpose: subcloning of 3C into pDB.His.MBP(V14)

Date:  2012-09-18

Notebook: 

Page:

Clone ID: 1375

Experimenters: Xiuli

Results

Cloning

09-19-2012 A.Restriction enzyme digestion: Vector V14 pDB.His.MBP and 1197 pDB.His.GST.3C with NdeI and XhoI, B.Gel purification (elute with 15 ul H2O), and ligation with T7 ligase at RT for2 hs (total volume in 30ul) C Transformation: DH5a + 20 ul ligation Re.

There is no colony on the plate. re-do the experiment again.

10/7/2012

Digest miniprep with Nde1 + Xho1

clones 1,2, 4,5 look ok.