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Vectors for Antibody Expression
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{| border="1" cellspacing="1" cellpadding="1" style="width: 647px;" |- | style="width: 51px;" | ID | style="width: 85px;" | Name | style="width: 69px;" | Promoter | style="width: 19px;" | Signal | style="width: 60px;" | Species | style="width: 55px;" | Isotype | style="width: 130px;" | MCS | style="width: 25px;" | Status | style="width: 115px;" | Note |- | style="width: 51px;" | 2492<br/> | style="width: 85px;" | pCMV5-mIgG2b-HC<br/> | style="width: 69px;" | CMV5 | style="width: 19px;" | none | style="width: 60px;" | mouse | style="width: 55px;" | IgG2b Heavy<br/> | style="width: 130px;" | BamH1 GGATCC-- Kpn1 GGTACC GT/AKTTAPS | style="width: 25px;" | 0 | style="width: 115px;" | not compatible with VH domain designed for pCEH0 |- | style="width: 51px;" | 2493<br/> | style="width: 85px;" | pCMV5-mIgG-kLC<br/> | style="width: 69px;" | CMV5 | style="width: 19px;" | none | style="width: 60px;" | mouse<br/> | style="width: 55px;" | kappa light<br/> | style="width: 130px;" | BamH1 GGATCC-- Kpn1 GGTACC GT/ADAAP | style="width: 25px;" | 0 | style="width: 115px;" | not compatible with VL domain designed for pCEL0<br/> |- | style="width: 51px;" | 3016<br/> | style="width: 85px;" | pCEL0<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | <br/> | style="width: 55px;" | light | style="width: 130px;" | BssH2 Nhe1 GCGCGC TGT ---- GCTAGC | style="width: 25px;" | 0 | style="width: 115px;" | backbone Nhe1 is compatible with Xba1 |- | style="width: 51px;" | 3017<br/> | style="width: 85px;" | pCEH0<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | <br/> | style="width: 55px;" | heavy | style="width: 130px;" | BsrG1 TGTaCActcc--Sal1, Kpn1, pvUII, Nhe1, HindIII, Not1 | style="width: 25px;" | 0 | style="width: 115px;" | backbone<br/> |- | style="width: 51px;" | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;">3020</span><br/> | style="width: 85px;" | pCEH0-mIgG1-HC<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | mouse | style="width: 55px;" | mIgG1 heavy | style="width: 130px;" | BsrG1 Nhe1<br/>TGTaCActcc ---GCTAGC ASSAKTTPP | style="width: 25px;" | 1 | style="width: 115px;" | VH domain |- | style="width: 51px;" | 3021<br/> | style="width: 85px;" | pCEL0-mIg-LCKappa<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | mouse | style="width: 55px;" | Ig Kappa | style="width: 130px;" | BssHII <span style="color:#0000FF;">KpnI</span> or Xho1 GCGCGCTGT-- GGtACC <span style="color:#0000FF;">GT</span>/K<span style="color:#FF0000;">LE</span>IKRADAAP | style="width: 25px;" | 1 | style="width: 115px;" | VH |- | style="width: 51px;" | 3025<br/> | style="width: 85px;" | pCEL0-rabbit-Ig-LKappa<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | rabbit | style="width: 55px;" | light kappa | style="width: 130px;" | BssHII --Kpn1 GCGCGC -- GGtACC GT/E V V V K G D | style="width: 25px;" | in pJet1.2 now, need to sublcone | style="width: 115px;" | <br/> |- | style="width: 51px;" | 3026 <br/> | style="width: 85px;" | pCEH0-rabbit-IgG-HC<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | rabbit | style="width: 55px;" | heavy | style="width: 130px;" | BsrG1--Nhe1 GCTAGC AS/SGQPKAP | style="width: 25px;" | in pJet1.2 now, need to sublcone<br/> | style="width: 115px;" | <br/> |- | style="width: 51px;" | 3027<br/> | style="width: 85px;" | pCEH0-mIgG2b-HC<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | mouse | style="width: 55px;" | IgG2b | style="width: 130px;" | BsrG1--Nhe1 AS/SAKTTPP | style="width: 25px;" | need to PCR from 2492 | style="width: 115px;" | <br/> |- | style="width: 51px;" | pFuse4<br/> | style="width: 85px;" | pFUSE-hIgG4-Fc1<br/> | style="width: 69px;" | hEF1-HTLV<br/> | style="width: 19px;" | yes | style="width: 60px;" | human | style="width: 55px;" | IgG4 | style="width: 130px;" | BamH1--Xba1 | style="width: 25px;" | 0 | style="width: 115px;" | <br/> |- | style="width: 51px;" | 3030<br/> | style="width: 85px;" | pCEH0-mIgG2a-HC<br/> | style="width: 69px;" | cag | style="width: 19px;" | yes | style="width: 60px;" | mouse | style="width: 55px;" | mIgG2a<br/> | style="width: 130px;" | BsrG1--Nhe1 AS/SAKTTPP | style="width: 25px;" | need to find mouse IgG2a clone | style="width: 115px;" | <br/> |- | style="width: 51px;" | 3096<br/> | style="width: 85px;" | pCEL0-L-clone3<br/> | style="width: 69px;" | CAG | style="width: 19px;" | yes | style="width: 60px;" | human | style="width: 55px;" | light kappa<br/> | style="width: 130px;" | BssHII--Nhe1 AgeI (entire kappa) | style="width: 25px;" | <br/> | style="width: 115px;" | <br/> |- | style="width: 51px;" | 3095<br/> | style="width: 85px;" | pCEH0-H-clone3<br/> | style="width: 69px;" | cag | style="width: 19px;" | yes | style="width: 60px;" | human | style="width: 55px;" | heavy | style="width: 130px;" | BsrG1<br/> | style="width: 25px;" | <br/> | style="width: 115px;" | <br/> |- | style="width: 51px;" | <br/> | style="width: 85px;" | <br/> | style="width: 69px;" | <br/> | style="width: 19px;" | <br/> | style="width: 60px;" | <br/> | style="width: 55px;" | <br/> | style="width: 130px;" | <br/> | style="width: 25px;" | <br/> | style="width: 115px;" | <br/> |} [[Category:Antibodies]]
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