Vectors for Antibody Expression

ID	Name	Promoter	Signal	Species	Isotype	MCS	Status	Note
2492	pCMV5-mIgG2b-HC	CMV5	none	mouse	IgG2b Heavy	BamH1 GGATCC-- Kpn1 GGTACC GT/AKTTAPS	0	not compatible with VH domain designed for pCEH0
2493	pCMV5-mIgG-kLC	CMV5	none	mouse	kappa light	BamH1 GGATCC-- Kpn1 GGTACC GT/ADAAP	0	not compatible with VL domain designed for pCEL0
3016	pCEL0	CAG	yes		light	BssH2                   Nhe1 GCGCGC TGT ---- GCTAGC	0	backbone Nhe1 is compatible with Xba1
3017	pCEH0	CAG	yes		heavy	BsrG1 TGTaCActcc--Sal1,  Kpn1, pvUII, Nhe1, HindIII, Not1	0	backbone
3020	pCEH0-mIgG1-HC	CAG	yes	mouse	mIgG1 heavy	BsrG1   Nhe1 TGTaCActcc ---GCTAGC  ASSAKTTPP	1	VH domain
3021	pCEL0-mIg-LCKappa	CAG	yes	mouse	Ig Kappa	BssHII             KpnI or Xho1  GCGCGCTGT-- GGtACC GT/KLEIKRADAAP	1	VH
3025	pCEL0-rabbit-Ig-LKappa	CAG	yes	rabbit	light kappa	BssHII  --Kpn1 GCGCGC -- GGtACC GT/E V V V K G D	in pJet1.2 now, need to sublcone
3026	pCEH0-rabbit-IgG-HC	CAG	yes	rabbit	heavy	BsrG1--Nhe1 GCTAGC AS/SGQPKAP	in pJet1.2 now, need to sublcone
3027	pCEH0-mIgG2b-HC	CAG	yes	mouse	IgG2b	BsrG1--Nhe1 AS/SAKTTPP	need to PCR from 2492
pFuse4	pFUSE-hIgG4-Fc1	hEF1-HTLV	yes	human	IgG4	BamH1--Xba1	0
3030	pCEH0-mIgG2a-HC	cag	yes	mouse	mIgG2a	BsrG1--Nhe1 AS/SAKTTPP	need to find mouse IgG2a clone
3096	pCEL0-L-clone3	CAG	yes	human	light kappa	BssHII--Nhe1            AgeI  (entire kappa)
3095	pCEH0-H-clone3	cag	yes	human	heavy	BsrG1