Editing Yijia LI 5 29 2018

'''Vector digestion'''

[[File:IMG 0858.JPG|RTENOTITLE]]

'''Subclone and Vector digestion'''

[[File:IMG 0860.JPG|RTENOTITLE]]

'''Subclone of 931'''

[[File:IMG 0859.JPG|RTENOTITLE]]

'''PCR of 994_995'''

[[File:IMG 0856.JPG|RTENOTITLE]]

'''PCR of 3192-F1__3196'''

[[File:IMG 0857.JPG|RTENOTITLE]]

'''PCR of 3200'''

[[File:IMG 0853.JPG|RTENOTITLE]]

'''PCR of 3197'''

[[File:IMG 0849.JPG|RTENOTITLE]]

'''PCR of 998_1000'''

[[File:IMG 0851.JPG|RTENOTITLE]]

'''PCR of 3195_3199'''

[[File:IMG 0852.JPG|RTENOTITLE]]

'''3194 PCR'''

[[File:IMG 0855.JPG|RTENOTITLE]]

'''cDNA PCR of AH10_FE2'''

[[File:IMG 0848.JPG|RTENOTITLE]]

'''Miniprep digestion of 984_931'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 984
| 2-5
| Xba1+Nhe1
| 957bp
| '''#6,7,,8 seems good'''
|-
| 931
| 1,6-8
| HindIII+Xho1
| 986bp
| '''#5 seems good'''
|}

[[File:IMG 0871.JPG|RTENOTITLE]]

'''Miniprep digestion of 2503_3122_3197_3191'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| lane
| Enzymes
| Size
| Note
|-
| 2503
| 1-4
| Nde1+Xho1
| 951bp
| #1,2 seems good
|-
| 3122
| 5-8
| Nde1+Xho1
| 1743bp
| #5,6,8 seems good
|-
| 3197
| 9-12
| BamH1+Nco1
| 1 band
| may not good
|-
| 3191
| 13-16
| BamH1+Nco1
| 722bp
| #14,15,16 seems good
|}

[[File:IMG 0872.JPG|RTENOTITLE]]

'''Miniprep digestion of 998_1000_994_995'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 998
| 1-4
| HindIII+Xho1
| <br/>
| <br/>
|-
| 1000
| 5-8
| HIndIII+Xho1
| <br/>
| <br/>
|-
| 994
| 9-12
| BamH1+Xho1
| <br/>
| <br/>
|-
| 995
| 13-16
| BamH1+Xho1
| <br/>
| <br/>
|}

'''''[[File:IMG 0873.JPG|RTENOTITLE]]'''''

'''Miniprep of 3199'''

[[File:IMG 0898.JPG|RTENOTITLE]]

'''Vector digestion'''

[[File:Vector digestion (2).JPG]]
[[Category:My ELN]]