Yijia LI 5 29 2018

Vector digestion

Subclone and Vector digestion

Subclone of 931

PCR of 994_995

PCR of 3192-F1__3196

PCR of 3200

PCR of 3197

PCR of 998_1000

PCR of 3195_3199

3194 PCR

cDNA PCR of AH10_FE2

Miniprep digestion of 984_931

#	Lane	Enzymes	Size	Note
984	2-5	Xba1+Nhe1	957bp	#6,7,,8 seems good
931	1,6-8	HindIII+Xho1	986bp	#5 seems good

Miniprep digestion of 2503_3122_3197_3191

#	lane	Enzymes	Size	Note
2503	1-4	Nde1+Xho1	951bp	#1,2 seems good
3122	5-8	Nde1+Xho1	1743bp	#5,6,8 seems good
3197	9-12	BamH1+Nco1	1 band	may not good
3191	13-16	BamH1+Nco1	722bp	#14,15,16 seems good

Miniprep digestion of 998_1000_994_995

#	Lane	Enzymes	Size	Note
998	1-4	HindIII+Xho1
1000	5-8	HIndIII+Xho1
994	9-12	BamH1+Xho1
995	13-16	BamH1+Xho1

Miniprep of 3199

Vector digestion