Hepes-NaOH buffer: Difference between revisions

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Latest revision as of 18:41, 17 December 2013

1M HEPES-NaOH pH 7.5

[edit]

700 ml ultrapure water
238.3 g HEPES
~5.5 g NaOH (sodium hydroxide) pellets to adjust pH to 7.5
to 1L w ultrapure water; sterilise by filtration

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