Hepes-NaOH buffer
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1M HEPES-NaOH pH 7.5
[edit]700 ml ultrapure water
238.3 g HEPES
~5.5 g NaOH (sodium hydroxide) pellets to adjust pH to 7.5
to 1L w ultrapure water; sterilise by filtration
700 ml ultrapure water
238.3 g HEPES
~5.5 g NaOH (sodium hydroxide) pellets to adjust pH to 7.5
to 1L w ultrapure water; sterilise by filtration