2134 pCMV5-ILK: Difference between revisions

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Latest revision as of 15:15, 5 April 2014

Cloning of 2134 pCMV5-ILK

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Purpose: Cloning of2134 pCMV5-ILK==

Date: 2014-4----

Notebook:0

Page:

Clone ID:2134

Experimenters:  shanyi

Results

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CLONING

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subclone from 2133 pT7A3-ILK using Nhe1-Not1 to 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP


Colony Screening

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Date: 2014_4_

Make miniprep, digestion with BamH1 + Kpn1

there are one BamHI and one KpnI site in ILK, so use BamH1 + Kpn1 to digest 2134,we will get four bands



Result intepretation/Summary

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Conclusion

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