2134 pCMV5-ILK
Jump to navigation
Jump to search
Cloning of 2134 pCMV5-ILK
[edit]Purpose: Cloning of2134 pCMV5-ILK==
Date: 2014-4----
Notebook:0
Page:
Clone ID:2134
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 2133 pT7A3-ILK using Nhe1-Not1 to 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP
Colony Screening
[edit]Date: 2014_4_
Make miniprep, digestion with BamH1 + Kpn1
there are one BamHI and one KpnI site in ILK, so use BamH1 + Kpn1 to digest 2134,we will get four bands
