2286 pCMV5-SBGFP: Difference between revisions

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Latest revision as of 18:15, 10 August 2014

Cloning of 2286 pCMV5-SBGFP

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Purpose: Cloning of2286 pCMV5-SBGFP==

Date: 2014-7--2014/8/1--

Notebook:0

Page:

Clone ID:2286

Experimenters:  shanyi

Results

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CLONING

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gene synthesis


digest with NheI+KpnI,clone into 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP




Colony Screening

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Date: 2014_8_1

Make miniprep, use NheI+KpnI to find positive clone

digest with NcoI



8/10/14

Transfection clone #2, #4 and #5  (row 3) are quite bright although frame shift



Result intepretation/Summary

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Conclusion

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