2286 pCMV5-SBGFP
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Cloning of 2286 pCMV5-SBGFP
[edit]Purpose: Cloning of2286 pCMV5-SBGFP==
Date: 2014-7--2014/8/1--
Notebook:0
Page:
Clone ID:2286
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
digest with NheI+KpnI,clone into 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP
Colony Screening
[edit]Date: 2014_8_1
Make miniprep, use NheI+KpnI to find positive clone
digest with NcoI
8/10/14
Transfection clone #2, #4 and #5 (row 3) are quite bright although frame shift
