2346 pET30b-T4-endo-VII-C6H: Difference between revisions

From ELN
Jump to navigation Jump to search
Excellgen (talk | contribs)
confirmed
1,723 edits
No edit summary
 
(No difference)

Latest revision as of 11:03, 26 September 2014

Cloning of 2346 pET30b-T4-endo-VII-C6H

[edit]

Purpose: Cloning of2346 pET30b-T4-endo-VII-C6H-==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2246

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from2166

T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac

T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag


DIGEST WITH Nde1 + Xho1


2


Colony Screening

[edit]

Date: 2014_8

Make miniprep, use  Nde1 + Xho1 to find positive clone




Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2346_pET30b-T4-endo-VII-C6H&oldid=13299"