2346 pET30b-T4-endo-VII-C6H

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Cloning of 2346 pET30b-T4-endo-VII-C6H

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Purpose: Cloning of2346 pET30b-T4-endo-VII-C6H-==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2246

Experimenters:  shanyi

Results

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CLONING

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PCR from2166

T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac

T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag


DIGEST WITH Nde1 + Xho1


2


Colony Screening

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Date: 2014_8

Make miniprep, use  Nde1 + Xho1 to find positive clone




Result intepretation/Summary

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Conclusion

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