758 pEc2-PacI: Difference between revisions
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Latest revision as of 17:35, 13 January 2015
Cloning of758 pEc2-PacI
[edit]Purpose: Cloning of758 pEc2-PacI==
Date: 2014-12_2015_1
Notebook:0
Page:
Clone ID:758
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
clone to 2015 using Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
2392digest.JPG)
Fix 758-2 and 758-3
[edit]pcr
fragment 1 using 758-2 as template
758-PacI-1+758-PacI-4
fragment 1 using 758-3 as template
758-PacI-5+758-PacI-12
clone to 2015 using Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
