758 pEc2-PacI

Cloning of758 pEc2-PacI

Purpose: Cloning of758 pEc2-PacI==

Date: 2014-12_2015_1

Notebook:0

Page:

Clone ID:758

Experimenters:  shanyi

Results

CLONING

gene synthesis

clone to 2015 using Nde1 + Xho1

Colony Screening

Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone

Fix 758-2 and 758-3

pcr

fragment 1 using 758-2 as template

758-PacI-1+758-PacI-4

fragment 1 using 758-3 as template

758-PacI-5+758-PacI-12

clone to 2015 using Nde1 + Xho1

Colony Screening

Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone

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