2437-pT7A30-Tat-NLS-Cas9

 Date:  2015-April

Clone ID: 2437

By: Xin Xu

Results: 

Clone TAT into vector 2332 pT7A30-Cas9 by NdeI +NcoI

PCR 1708 using

PCR on 1708 pT7A3-HTNCre

Digested the vector 2332 pT7A30-Cas9 by NdeI +NcoI

Ligation, transformation, screening:

 

PCR screening:

The primers used for screening was inside the gene: 2437-Tat-NLS-Nco1-R;2437-Tat-NLS-Nde1-F

All the clonies have the right PCR Product.

PCR screening again, using one primer was on the vector Pet-seq-F2 and one inside the gene 2437-Tat-NLS-Nco1-R

The colonies have a faint product. Also the -ve control also have the product. contaminated?

sent No3 to sequence.