2437-pT7A30-Tat-NLS-Cas9
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Date: 2015-April
Clone ID: 2437
By: Xin Xu
Results:
Clone TAT into vector 2332 pT7A30-Cas9 by NdeI +NcoI
PCR 1708 using
PCR on 1708 pT7A3-HTNCre
Digested the vector 2332 pT7A30-Cas9 by NdeI +NcoI
Ligation, transformation, screening:
PCR screening:
The primers used for screening was inside the gene: 2437-Tat-NLS-Nco1-R;2437-Tat-NLS-Nde1-F
All the clonies have the right PCR Product.
PCR screening again, using one primer was on the vector Pet-seq-F2 and one inside the gene 2437-Tat-NLS-Nco1-R
The colonies have a faint product. Also the -ve control also have the product. contaminated?
sent No3 to sequence.