2476 pEC2-TREC: Difference between revisions

From ELN
Jump to navigation Jump to search
Excellgen (talk | contribs)
confirmed
1,723 edits
No edit summary
 
(No difference)

Latest revision as of 15:04, 22 September 2016

Cloning of 2476 pEC2-TREC

[edit]

PCR using blood genomic DNA as template

RTENOTITLE


Digest with EcoR1 and Xho1, clone into EcoR1 and Xho1 digested pEC2.

PEC2 digestion 1.JPG



Colony Screening

[edit]

PCR screening

RTENOTITLE

Band1
 Band2
 Band13-18
No Template
 Primer
 2476

Make miniprep and digest with EcoR1 and Xho1.

RTENOTITLE

Retrieved from "https://eln.excellgen.com/index.php?title=2476_pEC2-TREC&oldid=15297"