2476 pEC2-TREC

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Cloning of 2476 pEC2-TREC

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PCR using blood genomic DNA as template

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Digest with EcoR1 and Xho1, clone into EcoR1 and Xho1 digested pEC2.

PEC2 digestion 1.JPG



Colony Screening

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PCR screening

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Band1
 Band2
 Band13-18
No Template
 Primer
 2476

Make miniprep and digest with EcoR1 and Xho1.

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