837 pMiniT-Hia5: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
(No difference)
|
Latest revision as of 13:21, 4 July 2017
6/23/2017
Colony PCR screening
6/24/17
Miniprep, digestion with EcoRV
miniprep purify by isopropanol precipitation
Miniprep purified by spin column
sequencing:
clone #2 has deletion mutation
clone #4 high background
837 clone #4, tranform DH5alpha with plasmid, make miniprep, digest with HindIII + Xba1
