837 pMiniT-Hia5
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6/23/2017
Colony PCR screening
6/24/17
Miniprep, digestion with EcoRV
miniprep purify by isopropanol precipitation
Miniprep purified by spin column
sequencing:
clone #2 has deletion mutation
clone #4 high background
837 clone #4, tranform DH5alpha with plasmid, make miniprep, digest with HindIII + Xba1
