3130 pCMV5-HA-VL-mIgKappa: Difference between revisions
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Latest revision as of 23:34, 4 February 2018
in pMiniT2 vector digestion with Xho1
date:1/31/2018 by Yijia
2/4/2018
3130 pCMV5-HA-VL-mIgKappa HA light
Vector Name: 2493 pCMV5-mIgG-kLC
PCR from 3011 pJet1.2-HA light chain
clone: 3011-L-1
3130-BamH1-R
atcatcggatccGCCGCCACCATGGACATGAGGGTC
3130-Kpn1-R
atcatcGGTACCccgcttcagttccagcttggttccgccgccaaatg
(catttggcggcggaaccaagctggaactgaagcggGGTACC gatgat)
Restriction Enzymes: BamH1-Kpn1
