3130 pCMV5-HA-VL-mIgKappa

in pMiniT2 vector digestion with Xho1

date:1/31/2018 by Yijia

2/4/2018

3130 pCMV5-HA-VL-mIgKappa HA light
Vector Name: 2493 pCMV5-mIgG-kLC
PCR from 3011 pJet1.2-HA light chain
clone: 3011-L-1 

3130-BamH1-R
atcatcggatccGCCGCCACCATGGACATGAGGGTC

3130-Kpn1-R
atcatcGGTACCccgcttcagttccagcttggttccgccgccaaatg
(catttggcggcggaaccaagctggaactgaagcggGGTACC gatgat)

Restriction Enzymes: BamH1-Kpn1