Transfection reagents commercial comparison

Commercial Transfection Reagents Comparison

Make different commercial transfection reagents (TR).

3 ul TR + 50ul DMEM without serum---->Mix A

1 ug DNA(pCMV-EGFP/ pAAV-CMV-mKate2) + 50ul DMEM without serum---->Mix B

Mix A + Mix B, incubate 15-20 min at room temperature

Add 100 ul mixture to transfect 293T cells in 12 well-plate.

24 hours after transfection, measured by SpectraMax M5.

EGFP plate 4: Temperature(°C)    1              2              3              4
                         26.2               675.324    580.481    602.805    731.362
                                              430.487    326.25      835.955    292.325
                                              646.244    262.669    429.855    744.585

MKATE2 plate6 Temperature(°C)  1             2                3              4
                         26.7                 83.5        39.662      617.972    109.662
                                                32.838      4.746       221.894    171.69
                                              483.829    157.576       37.564    332.106

48 hours after transfection, measured by SpectraMax M5.

EGFP plate 4: Temperature(°C)        1                2                3               4
                         26.7                 4496.206    2109.382    3187.399   1951.337
                                                  856.125      299.847     2564.626   1081.357
                                                 2655.973     990.368     1034.381    6137.053

MKATE2  plate 6:   Temperature(°C)  1             2                    3               4
                          26.1                  341.186      52.531        1695.635     325.586
                                                    80.697       10.806         478.409     449.453
                                                 1434.012      422.788         89.124     933.015

72 hours after transfection, measured by SpectraMax M5.

EGFP plate 4 Temperature(°C)        1                 2                 3               4
                         26                   7435.366     2311.691     5188.59     2759.943
                                                1120.156     275.718       4310.076   1187.699
                                                5695.792    2238.932      1402.173   9742.163

  

Max, 2011/11/10	LipoFectamine 2000	2020	Jakat
TKO	LT1	TurboFect	Attractene
Max 2011/11/10	Joe 3	XtremeGene 9	XtremeGene HP

48hours after tansfection, picture was taken on 11-15-2011.

Max, 2011/11/10	LipoFectamine 2000	2020	Jakat
TKO	LT1	TurboFect	Attractene
Max 2011/11/10	XFect	XtremeGene 9	XtremeGene HP

48hours after tansfection, picture was taken on 11-15-2011 (Plate 4).

Max, 2011/11/10	LipoFectamine 2000	2020	Jakat
TKO	LT1	TurboFect	Attractene
Max 2011/11/10	XFect	XtremeGene 9	XtremeGene HP

24hours after tansfection, picture was taken on 11-15-2011(Plate 6).

--xiaojing wang 15:11, 16 November 2011 (EST)