471-pAAV.CMV-FLPe: Difference between revisions
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Latest revision as of 13:06, 9 December 2011
Cloning of pAAV.CMV-FLPe
Purpose: to clone pAAV.CMV-FLPe by PCR
Date: 2011-12-7----
Notebook:Lu yingying
Page:
Clone ID:471
Experimenters: lu yingying
Results
gene synthesis
PCR1, 10 cycles
1 + 18
19 + 38
PCR2 mixture of PCR1
1 and 38 as primers, 25 cycles
digest with EcoR1 + BamH1
clone into EcoR1 + BamH1 digested pAAV.CMV-MCS, 254
ligate and transform STBL3
digest detect: EcoR1 + BamH1