471-pAAV.CMV-FLPe

Cloning of pAAV.CMV-FLPe

Purpose: to clone pAAV.CMV-FLPe by PCR

Date: 2011-12-7----

Notebook:Lu yingying

Page:

Clone ID:471

Experimenters: lu yingying
Results

gene synthesis
PCR1, 10 cycles
1 + 18
19 + 38

PCR2 mixture of PCR1

1 and 38 as primers, 25 cycles

digest with EcoR1 + BamH1
clone into EcoR1 + BamH1 digested pAAV.CMV-MCS, 254
ligate and transform STBL3

digest detect: EcoR1 + BamH1