1079-pLVX-loxP-sfGFP-loxP-Gluc

Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc

Purpose: Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc

Date: 2012-4-10--2012-4-15

Notebook:0

Page:

Clone ID:1079

Experimenters: yingying

Results

cloning

Date:2012-4-10

template:300pLentiGP-Gluc-PKAalpha

Primer-F Gluc-Xho1-F TAcAG G ctcgag gcc acc ATGGGAGTCAAAGTTCTGTTTG
Primer-R Gluc-Xba1-R (CAAGGGGGCCGGTGGTGACTAA TCT AGA tatgcat)

	band 1	band 2
	PCR product	1008pLVX-loxP-sfGFP-loxP
enzymes	Xho1+Xba1

Colony Screening

Date: 2012-4-13

Make miniprep and digest with Xho1+Xba1

need to sequence

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Conclusion

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