1079-pLVX-loxP-sfGFP-loxP-Gluc
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Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc
[edit]Purpose: Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc
Date: 2012-4-10--2012-4-15
Notebook:0
Page:
Clone ID:1079
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-4-10
template:300pLentiGP-Gluc-PKAalpha
Primer-F Gluc-Xho1-F TAcAG G ctcgag gcc acc ATGGGAGTCAAAGTTCTGTTTG
Primer-R Gluc-Xba1-R (CAAGGGGGCCGGTGGTGACTAA TCT AGA tatgcat)
| band 1 |
band 2 | |
| PCR product |
1008pLVX-loxP-sfGFP-loxP | |
| enzymes |
Xho1+Xba1 | |
Colony Screening
[edit]Date: 2012-4-13
Make miniprep and digest with Xho1+Xba1
need to sequence
Result intepretation/Summary
[edit]Conclusion
[edit]
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