PDB-His-NusA-StrepII: Difference between revisions
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Latest revision as of 18:25, 20 April 2011
Primer-F
StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa
Primer-R
StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa
anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.NusA. Transform Top 10 competent cells
mini-prep digest with Xba1+Pst1
--Paul.xu 17:24, 20 April 2011 (EST)