PDB-His-NusA-StrepII

Primer-F

StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa

Primer-R

StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa

anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.NusA. Transform Top 10 competent cells

mini-prep digest with Xba1+Pst1

--Paul.xu 17:24, 20 April 2011 (EST)