1528 pET28b.Amp.HC: Difference between revisions

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Cloning of 1528 pET28b.Amp.HC

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Purpose: Cloning of 1528 pET28b.Amp.HC

Date:Dec, 2012

Notebook: 

Page:

Clone ID: 1528

Experimenters: shanyi

Results

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Cloning

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digest pET28b with DraIII + Sap1, --> vector digest pRestA with DraIII+ Sap1--> 2171 bp fragment.

Colony Screening

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digest with BamHI and BsaI

Use sample1 .


1528-1 digest with ScaI + SapI

puc19 digest with ScaI + SapI+NdeI

Result intepretation/Summary

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Conclusion

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